EBioMedicine · 2019
Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia.
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Jiang Yang, Zhou Jinpeng, Zou Dan, Hou Dianqi, Zhang Haiying, Zhao Junshuang, Li Long, Hu Jiangfeng, Zhang Ye, Jing Zhitao
Аннотация
BACKGROUND: Glioma is the most common primary malignant tumor in the central nervous system with frequent hypoxia and angiogenesis. Limb-Bud and Heart (LBH) is a highly conserved transcription cofactor that participates in embryonic development and tumorigenesis.
METHODS: The conditioned media from LBH regulated human glioma cell lines and patient-derived glioma stem cells (GSCs) were used to treat the human brain microvessel endothelial cells (hBMECs). The function of LBH on angiogenesis were examined through methods of MTS assay, Edu assay, TUNEL assay, western blotting analysis, qPCR analysis, luciferase reporter assay and xenograft experiment.
FINDINGS: Our study found for the first time that LBH was overexpressed in gliomas and was associated with a poor prognosis. LBH overexpression participated in the angiogenesis of gliomas via the vascular endothelial growth factor A (VEGFA)-mediated extracellular signal-regulated kinase (ERK) signalling pathway in human brain microvessel endothelial cells (hBMECs). Rapid proliferation of gliomas can lead to tissue hypoxia and hypoxia inducible factor-1 (HIF-1) activation, while HIF-1 can directly transcriptionally regulate the expression of LBH and result in a self-reinforcing cycle.
INTERPRETATION: LBH may be a possible treatment target to break the vicious cycle in glioma treatment. : .
METHODS: The conditioned media from LBH regulated human glioma cell lines and patient-derived glioma stem cells (GSCs) were used to treat the human brain microvessel endothelial cells (hBMECs). The function of LBH on angiogenesis were examined through methods of MTS assay, Edu assay, TUNEL assay, western blotting analysis, qPCR analysis, luciferase reporter assay and xenograft experiment.
FINDINGS: Our study found for the first time that LBH was overexpressed in gliomas and was associated with a poor prognosis. LBH overexpression participated in the angiogenesis of gliomas via the vascular endothelial growth factor A (VEGFA)-mediated extracellular signal-regulated kinase (ERK) signalling pathway in human brain microvessel endothelial cells (hBMECs). Rapid proliferation of gliomas can lead to tissue hypoxia and hypoxia inducible factor-1 (HIF-1) activation, while HIF-1 can directly transcriptionally regulate the expression of LBH and result in a self-reinforcing cycle.
INTERPRETATION: LBH may be a possible treatment target to break the vicious cycle in glioma treatment. : .
Предметные рубрики MeSH
AnimalsCell Line, TumorDisease Models, AnimalExtracellular Signal-Regulated MAP KinasesFemaleGene ExpressionGene Knockdown TechniquesGliomaHeterograftsHumansHypoxiaImmunohistochemistryMiceNeoplasm GradingNeoplasm StagingNeovascularization, PathologicSignal TransductionTranscription FactorsVascular Endothelial Growth Factor A