Chemical constituents of the antiulcer purified fractions of Lindera reflexa Hemsl. and its quantitative analysis.

The root of Lindera reflexa Hemsl. (LR) is a folk Chinses herbal medicine that has been used to treat gastritis and peptic ulcers. In this study, three new stilbenes (1-3) and two known flavonoids (4 and 5) were isolated from the antiulcer purified fractions of LR. The chemical structures of the isolated compounds were characterized comprehensively based on the basis of extensive spectroscopic data. Absolute configurations of compounds 1, 2, and 3 were determined by ECD calculations. The cytotoxic activities of compounds 1-5 were evaluated by MTT assay. Compound 4 showed the strongest inhibitory effect on the proliferation of tumor cells lines MGC803 and SMMC-7721, with IC50 values of 2.65 and 4.13 μM, respectively. The quantitative analysis of 12 compounds of the antiulcer purified fractions of LR were carried out by using the reversed-phase high-performance liquid chromatography (HPLC) method. Within the test range, all calibration curves showed good linearity (R2 > 0.9993). The LOD, LOQ, specificity, precision, and accuracy of the method were verified. Therefore, the present study may provide a valuable method for quality control the antiulcer purified fractions of LR.