Zhurnal mikrobiologii, epidemiologii i immunobiologii · 2013
[Evaluation of expression of innate immunity component genes in mice leukocytes under the effect of synthetic ligands in vivo].
Аннотация на английскомСтатьяНазвание и аннотация: Оригинал на русском
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Labzhinov P A, Svitich O A, Gankovskaia L V, Zverev V V
Аннотация
AIM: Study the effect of synthetic ligands on the dynamics of TLR9 and BD-2 gene expression in vivo.
MATERIALS AND METHODS: Synthetic ligands were used whose sequence was homologous to DNA and RNA human viruses. At days 1, 7 and 28 blood sampling from BALB/c line mice was carried out. Determination of level of TLR9 and BD-2 gene expression was evaluated by using RT-PCR in real-time mode.
RESULTS: The following ligands had the maximum effectiveness: tgg-ccc-ccc-ttg-tgg-acc-gg, ccg-gtc-cac-aag-ggg-ggc-ca and tcg-tcg-ttt-tgt-cgt-tgt-cg. At day 1 after the administration of ligands a significant increase of TLR9 gene expression was observed (62, 18 and 13 times, respectively), but at the same time defensin gene expression changed insignificantly.
CONCLUSION: The approach developed may be used to evaluate the effect of these preparations on the system of innate immunity, namely TLR activation.
MATERIALS AND METHODS: Synthetic ligands were used whose sequence was homologous to DNA and RNA human viruses. At days 1, 7 and 28 blood sampling from BALB/c line mice was carried out. Determination of level of TLR9 and BD-2 gene expression was evaluated by using RT-PCR in real-time mode.
RESULTS: The following ligands had the maximum effectiveness: tgg-ccc-ccc-ttg-tgg-acc-gg, ccg-gtc-cac-aag-ggg-ggc-ca and tcg-tcg-ttt-tgt-cgt-tgt-cg. At day 1 after the administration of ligands a significant increase of TLR9 gene expression was observed (62, 18 and 13 times, respectively), but at the same time defensin gene expression changed insignificantly.
CONCLUSION: The approach developed may be used to evaluate the effect of these preparations on the system of innate immunity, namely TLR activation.