[Evaluation of expression of innate immunity component genes in mice leukocytes under the effect of synthetic ligands in vivo].

AIM: Study the effect of synthetic ligands on the dynamics of TLR9 and BD-2 gene expression in vivo.

MATERIALS AND

METHODS

Synthetic ligands were used whose sequence was homologous to DNA and RNA human viruses. At days 1, 7 and 28 blood sampling from BALB/c line mice was carried out. Determination of level of TLR9 and BD-2 gene expression was evaluated by using RT-PCR in real-time mode.

RESULTS

The following ligands had the maximum effectiveness: tgg-ccc-ccc-ttg-tgg-acc-gg, ccg-gtc-cac-aag-ggg-ggc-ca and tcg-tcg-ttt-tgt-cgt-tgt-cg. At day 1 after the administration of ligands a significant increase of TLR9 gene expression was observed (62, 18 and 13 times, respectively), but at the same time defensin gene expression changed insignificantly.

CONCLUSION

The approach developed may be used to evaluate the effect of these preparations on the system of innate immunity, namely TLR activation.