Future microbiology · 2026
Association of CRISPR/Cas system with integrons and antibiotic resistance in Kliebsiella pneumoniae.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
El-Sokkary Mohamed Mohamed Adel, Ali Israa Elsayed Hassan
Аннотация
AIMS: The interaction between the CRISPR/Cas system and drug resistance was investigated in this study.
METHODS: In this study, 24 isolates, identified as Klebsiella pneumoniae, were subjected to antimicrobial sensitivity screening and detecting the presence of cas, integrons, and antibiotic resistance genes.
RESULTS: The highest resistance level could be detected in ceftriaxone and amoxicillin by 79% and 70.8%, respectively, while the lowest was 16.7% for chloramphenicol. Additionally, 50% of all isolates exhibited multidrug resistance (MDR); however, extensive drug resistance (XDR) was present in 12.5%. Class 1 integrons with different sizes could be identified. sul1 and sul2 were the most resistance genes identified, followed by aacA4 and aac(3)-II and qnrS, with 58%, 50%, 41.7%, 33%, and 33%, respectively. Concerning CRISPR genes, cas3 could be identified in nine different isolates; however, cas1 could be detected in 11 different isolated strains. In case of CRISPR1, it was found in seven isolates (29.3%), while CRISPR2 could be identified in three different isolates (12.5%).
CONCLUSIONS: Antibiotic resistance genes were mostly correlated with CRISPR genes. Interestingly, BOX-PCR group A, with a lower number of CRISPR positive isolates (p = 0.046) had a limited number of detected genes, compared with other groups indicating a significant correlation between antibiotic resistance genes and cas genes (p = 0.0023).
METHODS: In this study, 24 isolates, identified as Klebsiella pneumoniae, were subjected to antimicrobial sensitivity screening and detecting the presence of cas, integrons, and antibiotic resistance genes.
RESULTS: The highest resistance level could be detected in ceftriaxone and amoxicillin by 79% and 70.8%, respectively, while the lowest was 16.7% for chloramphenicol. Additionally, 50% of all isolates exhibited multidrug resistance (MDR); however, extensive drug resistance (XDR) was present in 12.5%. Class 1 integrons with different sizes could be identified. sul1 and sul2 were the most resistance genes identified, followed by aacA4 and aac(3)-II and qnrS, with 58%, 50%, 41.7%, 33%, and 33%, respectively. Concerning CRISPR genes, cas3 could be identified in nine different isolates; however, cas1 could be detected in 11 different isolated strains. In case of CRISPR1, it was found in seven isolates (29.3%), while CRISPR2 could be identified in three different isolates (12.5%).
CONCLUSIONS: Antibiotic resistance genes were mostly correlated with CRISPR genes. Interestingly, BOX-PCR group A, with a lower number of CRISPR positive isolates (p = 0.046) had a limited number of detected genes, compared with other groups indicating a significant correlation between antibiotic resistance genes and cas genes (p = 0.0023).