Analytica chimica acta · 2025
Multiplex immunochromatographic assay based on triple-color aggregation-induced emission fluorescent microspheres with good biocompatibility for the simultaneous detection of veterinary drugs in aquatic products.
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Su Liu, Lai Xiaocui, Zhang Ganggang, Liu Cong, He Weihua, Zhang Gan, Lai Weihua, Deng Shengliang
Аннотация
BACKGROUND: The simultaneous presence of multiple veterinary drug residues in a single sample poses a significant challenge for food safety analysis. Compared with traditional single-target detection, multiplex detection can not only provide richer sample information to raise the detection efficiency and reduce the possibility of false positives and omissions, but also decrease sample volume, shorten detection time, and lower costs. The luminance of aggregation-induced emission fluorescent microspheres (AIEFMs) can be enhanced by loading more fluorophores. The AIEFMs exhibit more advantages such as bright colors, excellent monodispersity, and high photostability, making them widely applicable in food safety.
RESULTS: We successfully developed a multiplex immunochromatographic assay (ICA) using triple-color AIEFMs as signal labels for the simultaneous detection of three veterinary drugs: chloramphenicol (CAP), diethylstilbestrol (DES), and diazepam (DZP). AIEFMs with good biocompatibility were synthesized via a simple and quick one-pot method based on the self-aggregation of AIEgens and the coating of metal-polyphenol network formed through the coordination of proanthocyanidin and Fe3+ in a tetrahydrofuran/water mixed solution. The as-prepared AIEFMs were rapidly conjugated with antibodies to synthesize AIEFM probes without any coupling reagent, and the multiplex AIEFMs-ICA was established for the detection of CAP, DES, and DZP. Under optimal conditions, the detection limits for CAP, DES, and DZP were 1.67, 410, and 5 pg/mL, respectively. The average recoveries of these veterinary drugs in fish and shrimp samples ranged from 85.40 % to 113.15 %, demonstrating the reliability and stability of the multiplex AIEFMs-ICA.
SIGNIFICANCE: The multiplex AIEFMs-ICA exhibited excellent signal output capacity, high specificity, and remarkable sensitivity. In brief, the developed multiplex AIEFMs-ICA shows great potential in the on-site detection of veterinary drugs and other targets.
RESULTS: We successfully developed a multiplex immunochromatographic assay (ICA) using triple-color AIEFMs as signal labels for the simultaneous detection of three veterinary drugs: chloramphenicol (CAP), diethylstilbestrol (DES), and diazepam (DZP). AIEFMs with good biocompatibility were synthesized via a simple and quick one-pot method based on the self-aggregation of AIEgens and the coating of metal-polyphenol network formed through the coordination of proanthocyanidin and Fe3+ in a tetrahydrofuran/water mixed solution. The as-prepared AIEFMs were rapidly conjugated with antibodies to synthesize AIEFM probes without any coupling reagent, and the multiplex AIEFMs-ICA was established for the detection of CAP, DES, and DZP. Under optimal conditions, the detection limits for CAP, DES, and DZP were 1.67, 410, and 5 pg/mL, respectively. The average recoveries of these veterinary drugs in fish and shrimp samples ranged from 85.40 % to 113.15 %, demonstrating the reliability and stability of the multiplex AIEFMs-ICA.
SIGNIFICANCE: The multiplex AIEFMs-ICA exhibited excellent signal output capacity, high specificity, and remarkable sensitivity. In brief, the developed multiplex AIEFMs-ICA shows great potential in the on-site detection of veterinary drugs and other targets.