FREQUENCY OF OCCURRENCE OF POLYMORPHIC VARIANTS OF IL28B GENE AND GENOTYPES OF HEPATITIS C VIRUS IN POPULATION OF YAKUTIA: CLINICAL OUTCOMES.

AIM: Study clinical outcomes in patients with chronic hepatitis C depending on genotype of hepatitis C virus (HCV) and IL28B gene polymorphism.

MATERIALS AND

METHODS

592 indi- viduals Were examined, 75 ofthosehad HCVRNAgenotypes determined by PCR. Genotyping of single nucleotide polymorphisms (SNP) - rs12979860 (C/T) and rs8099917 (T/G) in IL28B gene was carried out by real-time PCR.

RESULTS

HCV RNA was detected in 72 examined residents of Yakutia. HCV lb genotype was determined in 74.2% of cases, 3a - in 11.4%, la and 2 - 5.7% each. Frequency of polymorph variant rs12979860 CC was 72.2%, CT - 27.8%, rs8099917 TT - 61.1%, TG - 23.2%.

CONCLUSION

Combination of HCV lb with polymorphic variants of IL28B'gene rs12979860 CC and rs8099917 CT showed a less aggressive course of the disease. On the other hand, HCV infection of individuals with geno- type 3a and polymorphism rs12979860 CC or rs809917 TT of IL28B gene showed a more severe clinical presentation. The presence ofpolyorirphvariants rs8099917 T/G and rs12979860 C/T showed more severe clinical outcomes of HCV infection (viral load up to 19035212 copies, cirrhosis with ascite, hepatocarcinoma).