Leukemia research · 1998
Analysis of megakaryocyte growth and development factor (thrombopoietin) effects on blast cell and megakaryocyte growth in myelodysplasia.
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Fontenay-Roupie M, Dupont J M, Picard F, Fichelson S, Botella A, Melle J, Guesnu M, Casadevall N, Dreyfus F, Bouscary D
Аннотация
Thrombocytopenia is a frequent feature of myelodysplastic syndromes (MDS) that could be improved by the use of recombinant human megakaryocyte growth and development factor (rHuMGDF). Using short-term liquid cultures and progenitor assays, we have found that rHuMGDF stimulated DNA synthesis and potentiated leukemic cluster growth of bone marrow mononuclear cells in 10/38 MDS cases (26%). Cytogenetically malignant colonies were detectable in rHuMGDF-stimulated cultures (n=3) by fluorescence in situ hybridization. rHuMGDF was able to stimulate CFU-MK formation in 45% of the samples tested. Finally, rHuMGDF-induced blast cell proliferation correlated with elevated expression of c-MPL, previously identified as a bad prognosis factor in MDS.
Предметные рубрики MeSH
Blast CrisisBlotting, NorthernCell Culture TechniquesCell DivisionClone CellsColony-Forming Units AssayDNAHematopoietic Stem CellsHumansIn Situ Hybridization, FluorescenceKaryotypingMegakaryocytesMethylcelluloseMyelodysplastic SyndromesNeoplasm ProteinsProto-Oncogene ProteinsRNAReceptors, CytokineReceptors, ImmunologicReceptors, ThrombopoietinRecombinant ProteinsThrombopoietin