[The relationship between content of malonic aldehyde, activity of glutathione peroxidase and polymorphism of gene GPX4 (718C/T) under chronic hepatitis C].

The study was carried out to evaluate concentration of malonic dialdehyde and activity of glutathione peroxidase in blood serum in their relationship with biochemical tests of functional conditions of liver; data of fibroelastography and polymorphism of gene GPX4 (718C/T) in patients with chronic hepatitis C. The sampling consisted of 100 patients with chronic hepatitis C. The control group included 80 healthy donors. The polymorphism of gene of glutathione peroxidase was analyzed with CFX-96 (Bio-Rad Laboratories, Inc., USA) amplifier using technique of polymerase chain reaction ("Sintol", Moscow). In patients with chronic hepatitis C a reliable increasing of concentration of malonic dialdehyde up to 3.2 times and decreasing of glutathione peroxidase up to 2.8 times was detected as compared with control group. The significant differences in rates of genotypes and alleles of gene GPX4 (718C/T) in patients with chronic hepatitis C and healthy persons were not detected. The malonic dialdehyde demonstrated direct reliable relationships with functional hepatic tests by activity of alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transferase. The activity of glutathione peroxidase had reverse reliable correlations with alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transferase (p=0.001, p=0.007, p=0.032) and with indicator of elasticity of liver according data of fibroelastography (r=-0.285, p=0.041) The minor allele T of gene GPX4 ()718C/T) reliably correlated with alanine aminotransferase, aspartate aminotransferase and malonic dialdehyde. The reverse relationship between allele T of gene GPX4 ()718C/T) and activity of glutathione peroxidase (r=-0.196, p=0.041) was established This occurrence indicates at negative effect of mutation in gene of glutathione peroxidase on functional activity of this enzyme. Under chronic hepatitis C, the activation of peroxidation of lipids and depression of activity of glutathione peroxidase are interrelated with severity of cytolysis, cholestasis, expression of hepatic fibrosis and polymorphism of gene GPX4 ()718C/T). Therefore, polymorphism of gene of glutathione peroxidase predisposes to more severe affection of liver and progression of chronic hepatitis C.