Study of pathogenic factors of e.coli isolated from patients with peritonitis.

OBJECTIVE

The aim: To study the biological properties of museum and clinical strains of E.coli isolated from patients with peritonitis.

PATIENTS AND

METHODS

Materials and methods: It was used 94 strains (clinical, museum and reference). The ability of E. coli to adhere was investigated by hemadhezive method to formal human erythrocytes of 0 (I) Rh-positive blood group. The study measured the ability of microorganisms to produce gelatinaze, caseinase, fibrinolysin, hemolysin. To control of the enzyme activity the positive and negative control with reference strains were used. Synchronisation of cultures activity before experiments was achieved by one-time effect of low temperature (+4 С) during 30 minutes.

RESULTS

Results

To investigate the pathogenic factors of E. coli we carried out determining of proteolytic, gelatinous, caseinous, fibrinolytic, haemolytic and adhesion properties.

CONCLUSION

Conclusions

In our investigation pathogen icspecies of Esherihies are virtually indistinguishable from representatives of normal microflora on its morphological, biochemical and cultural properties. During investigation of serological properties of selected of E. coli strains (n = 94) 65.8% of pathogenic serotypes were revealed. Moreover, all marked E. coli isolated from the abdominal cavity of children and adults, as well as museum strains related to enteropathogenic E. coli (O127: K63, O33: K-) and 1 - to enteroinvazive Escherichia coli (O144: K-).