Effect of Topical Application of Resveratrol Loaded Chitosan Nanoparticles on Chemically Induced Epithelial Dysplasia in Hamster Pouch.

OBJECTIVES

This research aimed to investigate the topical effect of Resveratrol (RV) loaded on chitosan nanoparticles (CS-NP) on chemically induced epithelial dysplasia in the hamster buccal pouch.

MATERIAL AND

METHODS

35 Syrian hamsters were used in this study; they were divided into 4 groups. Group A (n = 5) was the negative control group. 7,12-dimethylbenzo anthracene (DMBA) was applied topically for 6 weeks to induce oral epithelial dysplasia in the remaining 30 animals, then they were divided into group B (n = 10) as the positive control group, group C (n = 10) animals received CS-NP (0.85 mg/100 L) topically, and group D (n = 10) animals received RV-CS-NP (0.85 mg/100 L) topically for another 6 weeks. Serial sections of the buccal pouches from all animals were examined histologically, and immunohistochemical (IHC) detection of the Bcl-2 gene expression was assessed.

RESULTS

The results showed that treatment with RV loaded on CS-NPs had significantly reduced the incidence of epithelial dysplastic lesions induced by the carcinogen. Data analysis showed statistically significant differences (p < 0.001) in the expression levels of Bcl-2 through IHC between the treatment and positive control groups.

CONCLUSION

Topical resveratrol-loaded chitosan nanoparticles are promising chemopreventive therapy.