Archives of oral biology · 2011
Effects of cigarette smoke condensate on oral squamous cell carcinoma cells.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Allam Eman, Zhang Weiping, Al-Shibani Nouf, Sun Jun, Labban Nawaf, Song Fengyu, Windsor L Jack
Аннотация
OBJECTIVE: Epidemiological studies have reported that tobacco use is a major etiological factor for oral cancer. Several matrix metalloproteinases (MMPs) have been shown to play important roles in the invasion and metastasis of oral squamous cell carcinomas, especially MMP-2 and MMP-9. This study examined the effects of cigarette smoke condensate (CSC) on oral cancer cells.
DESIGN: Two oral squamous cell carcinoma cell lines, SCC-25 (metastatic) and CAL-27 (non-metastatic), were exposed to different concentrations of CSC and examined for their collagen degrading ability and MMP production using collagen degradation assays, zymograms and Western blots.
RESULTS: Exposure to CSC increased the collagen degrading ability of the metastasizing cell line (SCC-25) by a mechanism involving increased MMP-2 and MMP-9 production.
CONCLUSION: CSC increased the collagen degrading ability of SCC-25 by increasing the MMP-2 and MMP-9 protein levels. Continued cigarette smoking in oral cancer patients may result in decreased survival rates due to enhanced metastatic potential of the cancer cells.
DESIGN: Two oral squamous cell carcinoma cell lines, SCC-25 (metastatic) and CAL-27 (non-metastatic), were exposed to different concentrations of CSC and examined for their collagen degrading ability and MMP production using collagen degradation assays, zymograms and Western blots.
RESULTS: Exposure to CSC increased the collagen degrading ability of the metastasizing cell line (SCC-25) by a mechanism involving increased MMP-2 and MMP-9 production.
CONCLUSION: CSC increased the collagen degrading ability of SCC-25 by increasing the MMP-2 and MMP-9 protein levels. Continued cigarette smoking in oral cancer patients may result in decreased survival rates due to enhanced metastatic potential of the cancer cells.
Предметные рубрики MeSH
Blotting, WesternCarcinoma, Squamous CellCell Line, TumorCell ProliferationCell ShapeCollagen Type IEnzyme ActivationHumansIndicators and ReagentsL-Lactate DehydrogenaseMatrix Metalloproteinase 2Matrix Metalloproteinase 9SmokeTetrazolium SaltsTime FactorsTissue Inhibitor of Metalloproteinase-1Tissue Inhibitor of Metalloproteinase-2Tongue NeoplasmsNicotiana