Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2002
[Clone of a novel liver cancer associated gene and analysis of the secondary structures of the predicted protein].
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Wang Zhengxu, Hu Guifang, Wang Hongyang, Wu Mengchao
Аннотация
OBJECTIVE: To clone a novel liver cancer associated gene, and to explore the molecular basis of liver cancer genesis.
METHODS: Using mRNA differential display polymerase chain reaction (DDPCR) and screening the human placenta cDNA library, we got a full-length cDNA of the gene. We prepared and purified the GST fusion protein and the special polyclonal antibody, engaged in the Western blot and immunohistochemical staining analysis, and analyzed the second structures and predicted the function of the protein by the computer soft.
RESULTS: We have got a full-length cDNA of the liver cancer associated gene and identified that the full-length cDNA of the gene could be expressed in 293 eukaryocytes by Western blot assay. We localized the target protein in cytoplasm using the immunohistochemical staining methods, and found two SH3 binding domains and several protein kinase phosphorylation sites by analyzing the second structures.
CONCLUSIONS: We have got a novel full-length cDNA of human liver cancer associated gene.
METHODS: Using mRNA differential display polymerase chain reaction (DDPCR) and screening the human placenta cDNA library, we got a full-length cDNA of the gene. We prepared and purified the GST fusion protein and the special polyclonal antibody, engaged in the Western blot and immunohistochemical staining analysis, and analyzed the second structures and predicted the function of the protein by the computer soft.
RESULTS: We have got a full-length cDNA of the liver cancer associated gene and identified that the full-length cDNA of the gene could be expressed in 293 eukaryocytes by Western blot assay. We localized the target protein in cytoplasm using the immunohistochemical staining methods, and found two SH3 binding domains and several protein kinase phosphorylation sites by analyzing the second structures.
CONCLUSIONS: We have got a novel full-length cDNA of human liver cancer associated gene.
Предметные рубрики MeSH
Amino Acid SequenceCell Cycle ProteinsCell LineCloning, MolecularCytoplasmDNA, NeoplasmGene Expression ProfilingGene Expression Regulation, NeoplasticGene LibraryHumansLiver NeoplasmsMolecular Sequence DataNeoplasm ProteinsNuclear ProteinsPhosphorylationPolymerase Chain ReactionProtein Structure, SecondaryProteinsRecombinant ProteinsTrans-ActivatorsTranscription Factors