Zhonghua bing li xue za zhi = Chinese journal of pathology · 2007
[Clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue lymphoma].
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Jiang Wei, Li Gan-di, Li Lei, Tang Yuan, He Yan-mei
Аннотация
OBJECTIVE: To analyze the clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue (MALT) lymphoma.
METHODS: Six cases of MALT lymphoma with high-grade transformation were studied. Immunohistochemical study was carried out by EliVision using bcl-10 antibody. Reverse transcription-polymerase chain reaction was used to detect the presence of API2-MALT1 fusion gene transcripts. The target cells were selected by laser microdissection and studied by polymerase chain reaction and sequence analysis for rearrangement of immunoglobulin heavy chain gene.
RESULTS: In the 6 cases of MALT lymphoma with high-grade transformation, nuclear and cytoplasmic expression of bcl-10 was demonstrated in 1 case, while API2-MALT1 fusion gene was present in 2 cases. Identical fragments of rearranged immunoglobulin heavy chain gene were detected in both transformed and non-transformed components in each of the 6 cases, except for the differences at 2 nucleotide positions in N or D regions in 2 cases.
CONCLUSION: The tumor cells from both transformed and non-transformed components in MALT lymphoma derive from the same clone.
METHODS: Six cases of MALT lymphoma with high-grade transformation were studied. Immunohistochemical study was carried out by EliVision using bcl-10 antibody. Reverse transcription-polymerase chain reaction was used to detect the presence of API2-MALT1 fusion gene transcripts. The target cells were selected by laser microdissection and studied by polymerase chain reaction and sequence analysis for rearrangement of immunoglobulin heavy chain gene.
RESULTS: In the 6 cases of MALT lymphoma with high-grade transformation, nuclear and cytoplasmic expression of bcl-10 was demonstrated in 1 case, while API2-MALT1 fusion gene was present in 2 cases. Identical fragments of rearranged immunoglobulin heavy chain gene were detected in both transformed and non-transformed components in each of the 6 cases, except for the differences at 2 nucleotide positions in N or D regions in 2 cases.
CONCLUSION: The tumor cells from both transformed and non-transformed components in MALT lymphoma derive from the same clone.
Предметные рубрики MeSH
Adaptor Proteins, Signal TransducingAdultAgedB-Cell CLL-Lymphoma 10 ProteinBase SequenceCell NucleusCell Transformation, NeoplasticCloning, MolecularCytoplasmFemaleGene Rearrangement, B-Lymphocyte, Heavy ChainHumansLymphoma, B-Cell, Marginal ZoneMaleMiddle AgedOncogene Proteins, FusionPolymerase Chain Reaction