A possible involvement of the central endorphin system in the autoanalgesia induced by chronic administration of Freund's adjuvant solution in rats.

Chronic pain was induced in rats by daily injections of complete Freund's adjuvant into hind paws. Daily changes of pain threshold and endorphin (ED) content and their receptors in 4 divided parts: cortex, diencephalon-mesencephalon containing striatum (D-M), pons-medulla (P-M) and spinal cord, were measured. Decrease in pain responsiveness was observed in the adjuvant-injected group with concomitant increase of ED content in P-M and spinal cord. This decrease in pain responsiveness in the adjuvant-injected group was significantly different from that in the non-treated control group being partially reversed by naloxone. Furthermore, [3H]met-enkephalin binding sites increase in number in P-M of the adjuvant-injected group when maximal decrease of pain responsiveness was observed, returning to control level thereafter. Scatchard analysis revealed the increase of the low affinity binding site in P-M of the adjuvant-injected group. In cortex and D-M, on the other hand, ED content tended to decrease and no change was observed in number of [3H]met-enkephalin binding sites. These results indicate that the ED system in P-M and spinal cord may be more substantially involved in autoanalgesia than in cortex and D-M.