Head & neck · 2014
CD200: association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Jung Yuh-Seog, Vermeer Paola D, Vermeer Daniel W, Lee Sang-Jin, Goh Ah Ra, Ahn Hyun-Joo, Lee John H
Аннотация
BACKGROUND: The purpose of this study was to characterize the expression of CD200, a membrane protein that functions in immune evasion, to examine its correlations with cancer stem cell (CSC)-like features and analyze its response to chemotherapy and radiation in human papillomavirus (HPV)-positive (+) and negative (-) head and neck squamous cell carcinomas (HNSCCs).
METHODS: CD200 expression was analyzed in several HNSCC cell lines. CD200 was overexpressed in HPV(+) murine tonsil epithelial cells, its effects on Shh and Bmi-1 were examined in vitro, and tumor growth and response to chemoradiation were analyzed in vitro and in vivo.
RESULTS: CD200 was diversely expressed and consistently associated with expression of Bmi-1 and Shh. Overexpression of CD200 induced Bmi-1 and Shh. Tumors grew similarly between C57BL/6 and Rag1(-/-) C57BL/6 mice. CD200 expression enhanced the resistance to chemoradiation only in vivo.
CONCLUSION: CD200 was related to CSC features and modulates response to chemoradiation in vivo. Attenuating this might be a potential therapeutic strategy.
METHODS: CD200 expression was analyzed in several HNSCC cell lines. CD200 was overexpressed in HPV(+) murine tonsil epithelial cells, its effects on Shh and Bmi-1 were examined in vitro, and tumor growth and response to chemoradiation were analyzed in vitro and in vivo.
RESULTS: CD200 was diversely expressed and consistently associated with expression of Bmi-1 and Shh. Overexpression of CD200 induced Bmi-1 and Shh. Tumors grew similarly between C57BL/6 and Rag1(-/-) C57BL/6 mice. CD200 expression enhanced the resistance to chemoradiation only in vivo.
CONCLUSION: CD200 was related to CSC features and modulates response to chemoradiation in vivo. Attenuating this might be a potential therapeutic strategy.
Предметные рубрики MeSH
AnimalsAntigens, CDCarcinoma, Squamous CellCell Line, TumorCell ProliferationChemoradiotherapyFlow CytometryGene Expression Regulation, NeoplasticHead and Neck NeoplasmsHumansImmunoblottingMiceMice, Inbred C57BLNeoplasms, ExperimentalNeoplastic Stem CellsSquamous Cell Carcinoma of Head and NeckTransfectionTreatment Outcome