Early-stage immunoexpression of inflammatory, angiogenic, and survival markers in tongue epithelium of mice exposed to hookah smoke.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
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Pereira Aldini Beuting, Michels Arieli Carini, Pilati Sarah Freygang Mendes, Modolo Filipe, Martins Ana Paula Camargo, De Paula Caroline Busatta Vaz, Nagashima Seigo, Noronha Lúcia, Fonseca Ana Clara Prado, Turmina Ariane Jéssica Torres, De Meira Heloisa Franco, Ramos Luisa Gabriela Carneiro, Da Rosa Thaís Grupp, Eckhardt Thais Maria Dos Santos, Ignácio Sérgio Aparecido, Alanis Luciana Reis Azevedo, Souza Paulo Henrique Couto, Rached Rodrigo Nunes, Jham Bruno Correia, Carneiro Everdan, Dos Santos Emanuela Carla, Johann Aline Cristina Batista Rodrigues
Аннотация
MATERIALS AND METHODS: The sample consisted of Swiss mice (N = 20), female gender, aged 2 months, and approximately 25g each, four groups (n = 5) mice: group exposed to fresh air and groups exposed to hookah smoke for 7, 15, and 30 days. Tongues were embedded in paraffin. A tissue microarray was constructed, and immunohistochemistry was performed for Cyclooxygenase 2, NF-kappa B, Vascular Endothelial Growth Factor Receptor 1, and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) for apoptotic analysis. The positive and negative cells were quantified in the epithelium of the mid-dorsal tongue region. Kruskal-Wallis and Dunn tests was made.
RESULTS: The apoptotic index was higher at 30 days of smoke exposure (20.38% basal/ 19.63%/ suprabasal) compared to the group exposed to air (9.55%/ 11.88%), The expression of Vascular Endothelial Growth Factor Receptor 1 was higher at 30 days of smoke exposure (30.15%/ 38.15%) compared to the group exposed to air (18.25%/ 3.60%).
CONCLUSION: Hookah smoke induced greater apoptosis and increased expression of Vascular Endothelial Growth Factor Receptor 1 in the epithelium of the tongue at 30 days, potentially playing a role in the initial stages of carcinogenesis, in the early stages of hookah use.