Emerging infectious diseases · 2020
Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Harcourt Jennifer, Tamin Azaibi, Lu Xiaoyan, Kamili Shifaq, Sakthivel Senthil K, Murray Janna, Queen Krista, Tao Ying, Paden Clinton R, Zhang Jing, Li Yan, Uehara Anna, Wang Haibin, Goldsmith Cynthia, Bullock Hannah A, Wang Lijuan, Whitaker Brett, Lynch Brian, Gautam Rashi, Schindewolf Craig, Lokugamage Kumari G, Scharton Dionna, Plante Jessica A, Mirchandani Divya, Widen Steven G, Narayanan Krishna, Makino Shinji, Ksiazek Thomas G, Plante Kenneth S, Weaver Scott C, Lindstrom Stephen, Tong Suxiang, Menachery Vineet D, Thornburg Natalie J
Аннотация
The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.