BMC microbiology · 2025
Gastric cancer-associated bacteria exhibit distinct butyrate and pyruvate metabolism: a metabolomic analysis of F. nucleatum, N. subflava, and H. pylori.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Niikura Ryota, Hayakawa Yoku, Mukai Takahiro, Kato Yoichi, Yoshida Naofumi, Fujishiro Mitsuhiro
Аннотация
BACKGROUND: Butyrate and pyruvate are central metabolites in anaerobic microbial metabolism, with key roles in gastrointestinal physiology. We recently identified Fusobacterium nucleatum (F. nucleatum) and Neisseria subflava (N. subflava) as non-Helicobacter pylori (H. pylori) bacteria potentially involved in gastric cancer development. However, the metabolic pathways distinguishing F. nucleatum, N. subflava, and H. pylori remain poorly characterized.
METHODS: We performed capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) to profile the metabolic outputs of these three species under isolated culture conditions.
RESULTS: Distinct metabolic signatures were observed: F. nucleatum predominantly synthesized butyrate via the acetyl-CoA pathway, whereas N. subflava produced high levels of pyruvate and employed a cyclical route regenerating pyruvate from acetyl-CoA. In contrast, H. pylori lacked significant production of either metabolite.
CONCLUSIONS: This study delineates species-specific metabolic programs among gastric cancer-associated bacteria and highlights unique butyrate and pyruvate metabolism as a potential axis of microbe-host and microbe-microbe interaction in the gastric environment.
METHODS: We performed capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) to profile the metabolic outputs of these three species under isolated culture conditions.
RESULTS: Distinct metabolic signatures were observed: F. nucleatum predominantly synthesized butyrate via the acetyl-CoA pathway, whereas N. subflava produced high levels of pyruvate and employed a cyclical route regenerating pyruvate from acetyl-CoA. In contrast, H. pylori lacked significant production of either metabolite.
CONCLUSIONS: This study delineates species-specific metabolic programs among gastric cancer-associated bacteria and highlights unique butyrate and pyruvate metabolism as a potential axis of microbe-host and microbe-microbe interaction in the gastric environment.