Asian Pacific journal of cancer prevention : APJCP · 2015
Detection of BCR/ABL Fusion Gene by Hematological and Cytogenetical Analysis in Chronic Myeloid Leukemia Patients in Quetta, Pakistan.
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Tahira Bibi, Asif Muhammad, Khan Samiullah, Hussain Abrar, Shahwani Muhammad Naeem, Malik Arif, Inayatullah Syed, Iqbal Zafar, Rasool Mahmood
Аннотация
BACKGROUND: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells, caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known as the Philadelphia chromosome.
MATERIALS AND METHODS: A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms.
RESULTS: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 103/μl, hemoglobin 10.44 mg/dl, and platelets 288.6 103/μl were observed in this study.
CONCLUSIONS: In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.
MATERIALS AND METHODS: A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms.
RESULTS: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 103/μl, hemoglobin 10.44 mg/dl, and platelets 288.6 103/μl were observed in this study.
CONCLUSIONS: In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.
Предметные рубрики MeSH
AdolescentAdultAgedChildChild, PreschoolChromosomes, Human, Pair 22Chromosomes, Human, Pair 9Cytogenetic AnalysisFemaleFollow-Up StudiesFusion Proteins, bcr-ablHematologic TestsHumansIn Situ Hybridization, FluorescenceInfantInfant, NewbornLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMaleMiddle AgedNeoplasm StagingPhiladelphia ChromosomePrognosisSurvival RateTranslocation, GeneticYoung Adult