Diagnostic microbiology and infectious disease · 2019
Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.
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Kenmoe Sebastien, Tcharnenwa Clarisse, Monamele Gwladys C, Kengne Cyprien Nde, Ripa Mohamadou Njankouo, Whitaker Brett, Alroy Karen A, Balajee S Arunmozhi, Njouom Richard
Аннотация
INTRODUCTION: This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test.
METHODS: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.
RESULTS: The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51).
CONCLUSION: Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.
METHODS: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.
RESULTS: The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51).
CONCLUSION: Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.
Предметные рубрики MeSH
AdolescentAdultAgedAged, 80 and overCameroonChildChild, PreschoolClinical Laboratory TechniquesDiagnostic Tests, RoutineFemaleHumansInfantInfant, NewbornMaleMiddle AgedMolecular Diagnostic TechniquesNasopharynxReal-Time Polymerase Chain ReactionRespiratory Tract InfectionsReverse Transcriptase Polymerase Chain ReactionVirus Diseases