Fertility and sterility · 2007
Differential expression of microRNA species in human uterine leiomyoma versus normal myometrium.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Marsh Erica E, Lin Zhihong, Yin Ping, Milad Magdy, Chakravarti Debabrata, Bulun Serdar E
Аннотация
OBJECTIVE: To determine whether microRNAs (miRNAs) are differentially expressed in human leiomyoma versus matched myometrial tissue.
DESIGN: Microarray with real-time polymerase chain reaction (PCR) validation.
SETTING: Academic medical center.
PATIENT(S): Premenopausal subjects (n = 15), who were undergoing hysterectomies for leiomyoma-related symptoms.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Statistically differential expression of miRNAs in leiomyoma versus myometrium.
RESULT(S): Forty-six miRNA species were differentially expressed in leiomyoma versus normal myometrium. Of these, 19 were overexpressed and 27 were underexpressed in leiomyomas. The changes ranged from 1.2-fold to 11.8-fold. These findings were confirmed using real-time reverse transcriptase PCR for selected miRNAs (miRNAs 21, 34a, 125b, 139, and 323).
CONCLUSION(S): Our findings indicate that miRNAs are differentially expressed between human leiomyoma and matched myometrium. Given this differential expression, miRNAs may play a role in the pathogenesis of uterine leiomyoma and may serve as future therapeutic targets for the treatment of these tumors.
DESIGN: Microarray with real-time polymerase chain reaction (PCR) validation.
SETTING: Academic medical center.
PATIENT(S): Premenopausal subjects (n = 15), who were undergoing hysterectomies for leiomyoma-related symptoms.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Statistically differential expression of miRNAs in leiomyoma versus myometrium.
RESULT(S): Forty-six miRNA species were differentially expressed in leiomyoma versus normal myometrium. Of these, 19 were overexpressed and 27 were underexpressed in leiomyomas. The changes ranged from 1.2-fold to 11.8-fold. These findings were confirmed using real-time reverse transcriptase PCR for selected miRNAs (miRNAs 21, 34a, 125b, 139, and 323).
CONCLUSION(S): Our findings indicate that miRNAs are differentially expressed between human leiomyoma and matched myometrium. Given this differential expression, miRNAs may play a role in the pathogenesis of uterine leiomyoma and may serve as future therapeutic targets for the treatment of these tumors.