Journal of neurosurgery · 1998
Selective intraarterial gene delivery into a canine meningioma.
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Chauvet A E, Kesava P P, Goh C S, Badie B
Аннотация
OBJECT: The goal of this study was to evaluate gene delivery to a benign brain tumor.
METHODS: A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.
CONCLUSIONS: The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
METHODS: A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.
CONCLUSIONS: The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
Предметные рубрики MeSH
AdenoviridaeAnimalsColoring AgentsDNA, RecombinantDogsEscherichia coliEthmoid BoneFemaleGalactosidesGene Expression Regulation, EnzymologicGene Expression Regulation, NeoplasticGenes, ReporterGenetic TherapyGenetic VectorsIndolesInjections, Intra-ArterialMeningeal NeoplasmsMeningiomaTransduction, Geneticbeta-Galactosidase