Selective intraarterial gene delivery into a canine meningioma.

OBJECT: The goal of this study was to evaluate gene delivery to a benign brain tumor.

METHODS

A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.

CONCLUSIONS

The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.