Connexin 26 and basic fibroblast growth factor are expressed primarily in the subpial and subependymal layers in adult brain parenchyma: roles in stem cell proliferation and morphological plasticity?

The gap junction protein connexin 26 (Cx26) has been detected previously in the parenchyma of the developing brain and in the developing and adult meninges, but there is no clear evidence for the presence of this connexin in adult brain parenchyma. Confocal mapping of Cx26 through serial sections of the meningeal-intact rat brain with four antibodies revealed an intense Cx26 immunoreactivity in both parenchyma and extraparenchyma. In the extraparenchyma, a continuum of Cx26-immunoreactive puncta was observed throughout the three meningeal layers, the perineurium of cranial nerves, and meningeal projections into the brain, including sheaths of blood vessels and stroma of the choroid plexus. In the parenchyma, Cx26-immunoreactive puncta were located primarily in subependymal, subpial, and perivascular zones and were associated primarily with glial fibrillary acidic protein-positive (GFAP+) astrocytes, the nuclei of which are strongly immunoreactive for basic fibroblast growth factor (bFGF). Although it was found to a lesser extent than in astrocytes, bFGF immunoreactivity also was intense in the nuclei of meningeal fibroblasts. In addition, we have found a close correlation between the distribution of Cx26 and vimentin immunoreactivities in the meninges and their projections into the brain. We previously showed vimentin and S100beta immunoreactivities through a network of meningeal fibroblasts in the three layers of meninges, perivascular cells, and ependymocytes and in a population of astrocytes. The related topography of this network with GFAP+ astrocytes has also been demonstrated. Considering that connexin immunoreactivity may reflect the presence of functional gap junctions, the present results are consistent with our hypothesis that all of these various cell types may communicate in a cooperative network.