ANZ journal of surgery · 2016
Low prevalence of human papillomavirus in oral cavity squamous cell carcinoma in Queensland, Australia.
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Emmett Sarah, Jenkins Glenn, Boros Samuel, Whiteman David C, Panizza Benedict, Antonsson Annika
Аннотация
BACKGROUND: While human papillomavirus (HPV) is an accepted risk factor for oropharyngeal squamous cell carcinoma (SCC), its aetiological role in oral cavity SCC remains unclear. This study aimed to determine the HPV prevalence in an Australian population.
METHODS: DNA was extracted from 63 formalin-fixed paraffin-embedded tumour specimens histologically confirmed as SCC of the oral cavity, diagnosed during 2006-2012. Clinical data were extracted from medical records. HPV presence was determined by polymerase chain reaction. Positive samples were typed by sequencing. Immunohistochemistry was used to assess p16INK4A , p53, pRB, Ki67, Cyclin D1 and p21WAF1 expression.
RESULTS: Five of the 63 tumours (8%) were positive for HPV DNA (three HPV-16 positive and two HPV-18 positive). Two tumours overexpressed p16INK4A (3%) and one of these was also HPV positive. Overexpression of Cyclin D1 correlated significantly with tumour recurrence (P = 0.029) and death (P = 0.002).
CONCLUSIONS: This study has identified a low prevalence of high-risk HPV in Queensland, Australia.
METHODS: DNA was extracted from 63 formalin-fixed paraffin-embedded tumour specimens histologically confirmed as SCC of the oral cavity, diagnosed during 2006-2012. Clinical data were extracted from medical records. HPV presence was determined by polymerase chain reaction. Positive samples were typed by sequencing. Immunohistochemistry was used to assess p16INK4A , p53, pRB, Ki67, Cyclin D1 and p21WAF1 expression.
RESULTS: Five of the 63 tumours (8%) were positive for HPV DNA (three HPV-16 positive and two HPV-18 positive). Two tumours overexpressed p16INK4A (3%) and one of these was also HPV positive. Overexpression of Cyclin D1 correlated significantly with tumour recurrence (P = 0.029) and death (P = 0.002).
CONCLUSIONS: This study has identified a low prevalence of high-risk HPV in Queensland, Australia.
Предметные рубрики MeSH
AdultAgedAged, 80 and overAustraliaBiomarkers, TumorCarcinoma, Squamous CellCyclin D1FemaleHuman Papillomavirus DNA TestsHuman papillomavirus 16Human papillomavirus 18HumansMaleMiddle AgedMouthMouth NeoplasmsOropharyngeal NeoplasmsPapillomaviridaePapillomavirus InfectionsPrevalenceQueenslandRisk Factors