Head & neck · 2011
Selective culture of different types of human parotid gland cells.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chan Yen-Hui, Huang Tsung-Wei, Young Tai-Horng, Lou Pei-Jen
Аннотация
BACKGROUND: Advances in salivary gland tissue engineering can benefit patients diagnosed with xerostomia. Complexity of the gland explains the urgent demand for a reliable protocol to isolate and expand various gland cells that can be used for further study.
METHODS: Three cells with different morphologies were isolated from the same human parotid glands using different culture medium systems and then were identified by the expressions from mRNA to the protein level.
RESULTS: Among the 34 specimens, parotid gland acinar cells, myoepithelial cells, and fibroblasts expressing specific markers that belonged to individual cell types, were successfully isolated and expanded from 30 specimens without a complex mechanical process and expensive flow technique.
CONCLUSION: The proposed protocol is simple with a high success rate to culture various gland cells, making it highly promising for use in future tissue engineering studies.
METHODS: Three cells with different morphologies were isolated from the same human parotid glands using different culture medium systems and then were identified by the expressions from mRNA to the protein level.
RESULTS: Among the 34 specimens, parotid gland acinar cells, myoepithelial cells, and fibroblasts expressing specific markers that belonged to individual cell types, were successfully isolated and expanded from 30 specimens without a complex mechanical process and expensive flow technique.
CONCLUSION: The proposed protocol is simple with a high success rate to culture various gland cells, making it highly promising for use in future tissue engineering studies.
Предметные рубрики MeSH
Analysis of VarianceBlotting, WesternCell Culture TechniquesCell ProliferationCells, CulturedEpithelial CellsFemaleFibroblastsHumansImmunohistochemistryMaleMicroscopy, Electron, TransmissionMicroscopy, FluorescenceParotid GlandReverse Transcriptase Polymerase Chain ReactionSampling StudiesSensitivity and SpecificityTissue EngineeringXerostomia