Obesity research · 2005
Triglyceride-lowering effect of respiratory uncoupling in white adipose tissue.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Rossmeisl Martin, Kovar Jan, Syrovy Ivo, Flachs Pavel, Bobkova Dagmar, Kolar Frantisek, Poledne Rudolf, Kopecky Jan
Аннотация
OBJECTIVE: Hypolipidemic drugs such as bezafibrate and thiazolidinediones are known to induce the expression of mitochondrial uncoupling proteins (UCPs) in white adipose tissue. To analyze the potential triglyceride (TG)-lowering effect of respiratory uncoupling in white fat, we evaluated systemic lipid metabolism in aP2-Ucp1 transgenic mice with ectopic expression of UCP1 in adipose tissue.
RESEARCH METHODS AND PROCEDURES: Hemizygous and homozygous transgenic mice and their nontransgenic littermates were fed chow or a high-fat diet for up to 3 months. Total TGs, nonesterified fatty acids, and the composition of plasma lipoproteins were analyzed. Hepatic TG production was measured in mice injected with Triton WR1339. Uptake and the use of fatty acids were estimated by measuring adipose tissue lipoprotein lipase activity and fatty acid oxidation, respectively. Adipose tissue gene expression was assessed by quantitative reverse transcriptase-polymerase chain reaction.
RESULTS: Transgene dosage and the high-fat diet interacted to markedly reduce plasma TGs. This was reflected by decreased concentrations of very-low-density lipoprotein particles in the transgenic mice. Despite normal hepatic TG secretion, the activity of lipoprotein lipase in epididymal fat was enhanced by the high-fat diet in the transgenic mice in a setting of decreased re-esterification and increased in situ fatty acid oxidation.
DISCUSSION: Respiratory uncoupling in white fat may lower plasma lipids by enhancing their in situ clearance and catabolism.
RESEARCH METHODS AND PROCEDURES: Hemizygous and homozygous transgenic mice and their nontransgenic littermates were fed chow or a high-fat diet for up to 3 months. Total TGs, nonesterified fatty acids, and the composition of plasma lipoproteins were analyzed. Hepatic TG production was measured in mice injected with Triton WR1339. Uptake and the use of fatty acids were estimated by measuring adipose tissue lipoprotein lipase activity and fatty acid oxidation, respectively. Adipose tissue gene expression was assessed by quantitative reverse transcriptase-polymerase chain reaction.
RESULTS: Transgene dosage and the high-fat diet interacted to markedly reduce plasma TGs. This was reflected by decreased concentrations of very-low-density lipoprotein particles in the transgenic mice. Despite normal hepatic TG secretion, the activity of lipoprotein lipase in epididymal fat was enhanced by the high-fat diet in the transgenic mice in a setting of decreased re-esterification and increased in situ fatty acid oxidation.
DISCUSSION: Respiratory uncoupling in white fat may lower plasma lipids by enhancing their in situ clearance and catabolism.
Предметные рубрики MeSH
Adipose TissueAnimalsCarrier ProteinsDNA-Binding ProteinsDietary FatsEpididymisFatty AcidsFatty Acids, NonesterifiedGene ExpressionHomozygoteIon ChannelsKetone BodiesLipoprotein LipaseLipoproteinsLiverMaleMembrane ProteinsMiceMice, TransgenicMitochondriaMitochondrial ProteinsOxidation-ReductionPhosphoenolpyruvate Carboxykinase (GTP)Reverse Transcriptase Polymerase Chain ReactionTranscription Factor AP-2Transcription FactorsTriglyceridesUncoupling Protein 1