Cryo letters · 2016
Vitrification-based cryopreservation of protocorm-like bodies of an endangered lady's slipper orchid: Paphiopedilum niveum (Rchb.f.) Stein.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chaireok Supornchai, Thammasiri Kanchit, Meesawat Upatham
Аннотация
BACKGROUND: Paphiopedilum niveum (Rchb.f.) Stein, an endangered lady's slipper orchid, is listed in CITES Appendix I and thus requires conserving.
OBJECTIVE: The study aimed to cryopreserve protocorm-like bodies (PLBs) by a vitrification technique.
MATERIALS AND METHODS: Four-month-old PLBs were precultured in modified Vacin and Went liquid medium with various sucrose concentrations for 24 h and 5 d with daily increasing sucrose concentrations followed by different exposure times to plant vitrification solution 2 (PVS2).
RESULTS: Precultured PLBs in 0.75 M sucrose for 5 d with stepwise increase of sucrose and PVS2 for 90 min provided the highest viability after drying to low moisture content. The cryopreserved PLBs exhibited fewer dark stained nuclei and accumulated starch grains than those of the control. Cryopreserved PLBs had some shrunken epidermal cells, no change in ploidy level, a survival level of 22 % and the regenerated plantlets grew well.
CONCLUSION: This study is the first report evaluating the success of cryopreserved PLBs of P. niveum via a vitrification protocol.
OBJECTIVE: The study aimed to cryopreserve protocorm-like bodies (PLBs) by a vitrification technique.
MATERIALS AND METHODS: Four-month-old PLBs were precultured in modified Vacin and Went liquid medium with various sucrose concentrations for 24 h and 5 d with daily increasing sucrose concentrations followed by different exposure times to plant vitrification solution 2 (PVS2).
RESULTS: Precultured PLBs in 0.75 M sucrose for 5 d with stepwise increase of sucrose and PVS2 for 90 min provided the highest viability after drying to low moisture content. The cryopreserved PLBs exhibited fewer dark stained nuclei and accumulated starch grains than those of the control. Cryopreserved PLBs had some shrunken epidermal cells, no change in ploidy level, a survival level of 22 % and the regenerated plantlets grew well.
CONCLUSION: This study is the first report evaluating the success of cryopreserved PLBs of P. niveum via a vitrification protocol.