Archives of otolaryngology--head & neck surgery · 2003
Detection of Helicobacter pylori and its CagA gene in tonsil and adenoid tissues by PCR.
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Cirak Meltem Yalinay, Ozdek Ali, Yilmaz Dicle, Bayiz Unal, Samim Erdal, Turet Sevgi
Аннотация
OBJECTIVE: To determine the presence of Helicobacter pylori and, if detected, the prevalence of the CagA gene in adenotonsillectomy specimens by polymerase chain reaction (PCR).
DESIGN: A prospective clinical trial.
SETTING: Tertiary referral center.
PATIENTS AND METHODS: The study population comprised 23 patients who had undergone adenoidectomy, tonsillectomy, or adenotonsillectomy under local or general anesthesia. Helicobacter pylori DNA was extracted from 3-mm-diameter tissue samples obtained from each tonsil and adenoid tissue specimens. The amplifications were performed for the 16S ribosomal RNA (rRNA) and CagA genes of H pylori in the samples of which H pylori DNA was detected.
RESULTS: In examining all the samples, 7 (30%) of 23 patients were shown to be positive for H pylori DNA, 5 (71%) of whom also possessed the CagA gene.
CONCLUSIONS: Tonsil and adenoid tissues may be an ecological niche of the mouth without regard to transient or permanent colonization. Oral-oral transmission may be a possible mode of spread of H pylori.
DESIGN: A prospective clinical trial.
SETTING: Tertiary referral center.
PATIENTS AND METHODS: The study population comprised 23 patients who had undergone adenoidectomy, tonsillectomy, or adenotonsillectomy under local or general anesthesia. Helicobacter pylori DNA was extracted from 3-mm-diameter tissue samples obtained from each tonsil and adenoid tissue specimens. The amplifications were performed for the 16S ribosomal RNA (rRNA) and CagA genes of H pylori in the samples of which H pylori DNA was detected.
RESULTS: In examining all the samples, 7 (30%) of 23 patients were shown to be positive for H pylori DNA, 5 (71%) of whom also possessed the CagA gene.
CONCLUSIONS: Tonsil and adenoid tissues may be an ecological niche of the mouth without regard to transient or permanent colonization. Oral-oral transmission may be a possible mode of spread of H pylori.