Suppression of experimental autoimmune uveitis in guinea-pigs by inhibition of phospholipase A2.

Retinal S-antigen mixed with complete Freund's adjuvant was used to induce experimental autoimmune uveitis (EAU) in guinea-pigs. Guinea-pigs receiving no treatment, was compared with test animals which received topically and systemically administered KLM-583B, a phospholipase A2 (PLA2) inhibitor, or subcutaneous (sub. cut) and topical corticosteroid treatment, as well as a test group which received cyclosporin A suc. cut. The best clinical suppression of EAU was obtained in the group treated suc. cut with KLM-538B. Steroids also suppressed the inflammation in the eyes but was not as effective as KLM-583B or cyclosporine A. PLA 2 activity in the aqueous humour and the myeloperoxidase (MPO) levels measured from iris-ciliary body were significantly lower in the groups treated suc. cut. with KLM-583B or cyclosporin A. Guinea-pigs treated suc. cut. with KLM-583B and cyclosporin A had the lowest antiserum titres to retinal S-antigen.