Acta oto-laryngologica · 2014
Increased expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNA in chronic otitis media with cholesteatoma.
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Kim Myung Gu, Park Dong Choon, Oh In-Hwan, Kim Young Il, Choi Sun A, Jung Su Young, Kang Ho Min, Yeo Seung Geun
Аннотация
CONCLUSION: The increased expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNAs indicates that these pattern recognition receptors are involved in the pathogenesis of cholesteatoma.
OBJECTIVE: Changes in expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. We therefore assessed the levels of expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNAs associated with innate immune responses in patients with cholesteatoma.
METHODS: Cholesteatoma specimens were collected from 23 patients diagnosed with acquired cholesteatoma from August 2010 to July 2012. The posterior auricular skin of each patient was used as control. The levels of expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNA were assessed quantitatively using real-time RT-PCR and correlated with sex, hearing level, the presence of bacteria, and the need for repeat surgery.
RESULTS: The levels of expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNAs were significantly higher in cholesteatoma than in control skin samples (p < 0.05 each). However, mRNA abundance was not associated with patient sex, hearing level, presence of bacteria or history of reoperation (p > 0.05 each).
OBJECTIVE: Changes in expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. We therefore assessed the levels of expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNAs associated with innate immune responses in patients with cholesteatoma.
METHODS: Cholesteatoma specimens were collected from 23 patients diagnosed with acquired cholesteatoma from August 2010 to July 2012. The posterior auricular skin of each patient was used as control. The levels of expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNA were assessed quantitatively using real-time RT-PCR and correlated with sex, hearing level, the presence of bacteria, and the need for repeat surgery.
RESULTS: The levels of expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNAs were significantly higher in cholesteatoma than in control skin samples (p < 0.05 each). However, mRNA abundance was not associated with patient sex, hearing level, presence of bacteria or history of reoperation (p > 0.05 each).
Предметные рубрики MeSH
Adaptor Proteins, Signal TransducingAdolescentAdultAgedAntigens, CDB-Cell CLL-Lymphoma 10 ProteinChildCholesteatoma, Middle EarFemaleHepatitis A Virus Cellular Receptor 2HumansLectins, C-TypeMaleMembrane GlycoproteinsMembrane ProteinsMiddle AgedMinor Histocompatibility AntigensOtitis MediaReceptors, Cell SurfaceReceptors, ImmunologicTriggering Receptor Expressed on Myeloid Cells-1Young Adult