Age- and gender-related changes in the cochlear sympathetic system of the rat.

The sympathetic innervation projecting to the cochlea plays an important role in the auditory function, there is, however, no information about whether it is altered with advancing age. High performance liquid chromatography coupled to electrochemical detection was used to quantify both basal and noise-induced concentrations of norepinephrine (NE) in the rat cochlea. The cochlear concentration of NE was found to be independent of age in adult (3-12 months old) and aged (19 and 24 months old) males and the adult females. However, the concentrations of NE increased in aged females with respect to the younger ones, which suggests an increase in NE synthesis and a reduced NE release. Thus, a prominent gender effect emerged from this study, since the NE cochlear concentration was lower in adult females than in males, but tended to be the same level in aged animals. These modifications could be related to dramatic hormonal changes occurring in females with advancing age.