Neuromodulation : journal of the International Neuromodulation Society · 2026
High-Frequency Gastric Electrical Stimulation Selectively Remodels Nitrergic Neurons in the Gastric Antrum of Healthy Beagles.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Huang Bo, Fu Mingyu, Yang Heer, Yang Xueqian, Wu Xin, Zhao Yang, Fei Guijun, Li Jianing, Hu Chunhua, Tang Pan, Fang Xiucai
Аннотация
OBJECTIVES: The study aimed to investigate the effects and mechanisms of high-frequency short-pulse gastric electrical stimulation (GES) on gastric emptying and specific myenteric neurons of the gastric antrum in healthy beagle dogs.
MATERIALS AND METHODS: Four healthy adult dogs were implanted with gastric electrical devices, randomly divided into two groups, and given high-frequency short-pulse GES and sham GES (SGES) separately for four weeks in a crossover design, with a three-week washout period. The GES parameters were set 5 mA, 15 Hz, 0.33 ms, cycle on 0.2 s and off 3 s, and the SGES group received no stimulation. Food and water intake, body weight, and gastric emptying scintigraphy were evaluated before and after stimulation. The total neurons, choline acetyltransferase (ChAT), vasoactive intestinal peptide (VIP), neuronal nitric oxide synthase (nNOS), and calretinin (CALR)-immunoreactivity (IR) positive neurons were measured by indirect immunofluorescence double staining in the gastric antral specimens.
RESULTS: After four weeks of GES, dogs were shown significantly higher relative food intake and water intake, along with greater median weight gain compared with the SGES group. GES produced no significant effect on gastric emptying. The total number of antral myenteric neurons in full-thickness biopsies remained comparable between two groups; the proportion of nNOS-IR neurons was significantly higher in GES group than in SGES group (34.9% ± 1.7% vs 27.8% ± 1.9%, p = 0.004). Conversely, the proportion of VIP-IR neurons was lower in GES group than in SGES group (62.5% ± 1.5% vs 65.0% ± 1.8%, p = 0.019). No significant differences were observed in the proportions of ChAT-IR or CALR-IR neurons.
CONCLUSIONS: High-frequency short-pulse GES selectively increased the proportion of gastric nitrergic (nNOS-IR) neurons and improved feeding tolerance without altering gastric emptying in healthy dogs. This finding indicates that inhibitory nitrergic neuronal remodeling constitutes a key mechanism underlying GES, probably enhancing gastric accommodation.
MATERIALS AND METHODS: Four healthy adult dogs were implanted with gastric electrical devices, randomly divided into two groups, and given high-frequency short-pulse GES and sham GES (SGES) separately for four weeks in a crossover design, with a three-week washout period. The GES parameters were set 5 mA, 15 Hz, 0.33 ms, cycle on 0.2 s and off 3 s, and the SGES group received no stimulation. Food and water intake, body weight, and gastric emptying scintigraphy were evaluated before and after stimulation. The total neurons, choline acetyltransferase (ChAT), vasoactive intestinal peptide (VIP), neuronal nitric oxide synthase (nNOS), and calretinin (CALR)-immunoreactivity (IR) positive neurons were measured by indirect immunofluorescence double staining in the gastric antral specimens.
RESULTS: After four weeks of GES, dogs were shown significantly higher relative food intake and water intake, along with greater median weight gain compared with the SGES group. GES produced no significant effect on gastric emptying. The total number of antral myenteric neurons in full-thickness biopsies remained comparable between two groups; the proportion of nNOS-IR neurons was significantly higher in GES group than in SGES group (34.9% ± 1.7% vs 27.8% ± 1.9%, p = 0.004). Conversely, the proportion of VIP-IR neurons was lower in GES group than in SGES group (62.5% ± 1.5% vs 65.0% ± 1.8%, p = 0.019). No significant differences were observed in the proportions of ChAT-IR or CALR-IR neurons.
CONCLUSIONS: High-frequency short-pulse GES selectively increased the proportion of gastric nitrergic (nNOS-IR) neurons and improved feeding tolerance without altering gastric emptying in healthy dogs. This finding indicates that inhibitory nitrergic neuronal remodeling constitutes a key mechanism underlying GES, probably enhancing gastric accommodation.