Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2026
[Impact of icariin on the differentiation towards dentinogenesis and angiogenesis, and inflammatory regulation of stem cells from human exfoliated deciduous teeth].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Jin Yu, Dong Liying, Yuan Xiaojing, Song Rui, Yuan Zuoying, Zhao Yuming
Аннотация
OBJECTIVE: To explore the effects of icariin (ICA) on the proliferation and migration of stem cells from human exfoliated deciduous teeth (SHED), as well as its impact on dentinogenic and angiogenic differentiation in vitro, and its anti-inflammatory effects.
METHODS: (1) SHED cells were cultured in vitro, with a blank control group for the culture medium, a blank control group for DMSO, and different concentrations of ICA intervention groups (0.1, 1, 10, 20, 50 μmol/L), and cell viability was detected using the CCK-8 method. (2) Quantitative real-time PCR (RT-qPCR) was used to detect the gene expression levels of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1) and vascular endothelial growth factor A (VEGFA) in each group of cells. (3) Western blotting was used to detect the protein levels of DSPP, DMP1. (4) The scratch healing assay was used to test cell migration ability. (5) Lipopolysaccharide (LPS) was used to stimulate SHED cells to construct an in vitro inflammatory model of SHED, and RT-qPCR was used to detect the gene expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8) in each group of cells.
RESULTS: (1) In the 1, 10 μmol/L ICA intervention group, the proliferation ability of SHED cells was significantly enhanced; in the 0.1 μmol/L ICA intervention group, there was no significant change in the proliferation ability of SHED cells, and in the 20, 50 μmol/L ICA intervention group, the proliferation ability of SHED cells was significantly reduced. (2) In the 0.1, 1, 10 μmol/L ICA intervention group, the cell wound healing speed was faster. (3) In the 0.1, 1, 10 μmol/L ICA intervention group, the gene expression levels of DSPP and DMP1 were higher, and the protein expression levels of DSPP and DMP1 were higher in the 0.1 μmol/L ICA intervention group. (4) In the 1, 10 μmol/L ICA intervention group, the gene expression level of VEGFA was higher, and it had stronger tubular formation ability. (5) The expression of pro-inflammatory cytokines IL-1β, IL-6, TNF-α and IL-8 in the SHED cells in the 0.1, 1, 10 μmol/L ICA intervention group was significantly reduced.
CONCLUSION: Suitable concentrations of ICA can promote the proliferation, migration and odontoblast-like differentiation of SHED cells, enhance their angiogenic ability, and have a certain anti-inflammatory effect.
METHODS: (1) SHED cells were cultured in vitro, with a blank control group for the culture medium, a blank control group for DMSO, and different concentrations of ICA intervention groups (0.1, 1, 10, 20, 50 μmol/L), and cell viability was detected using the CCK-8 method. (2) Quantitative real-time PCR (RT-qPCR) was used to detect the gene expression levels of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1) and vascular endothelial growth factor A (VEGFA) in each group of cells. (3) Western blotting was used to detect the protein levels of DSPP, DMP1. (4) The scratch healing assay was used to test cell migration ability. (5) Lipopolysaccharide (LPS) was used to stimulate SHED cells to construct an in vitro inflammatory model of SHED, and RT-qPCR was used to detect the gene expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8) in each group of cells.
RESULTS: (1) In the 1, 10 μmol/L ICA intervention group, the proliferation ability of SHED cells was significantly enhanced; in the 0.1 μmol/L ICA intervention group, there was no significant change in the proliferation ability of SHED cells, and in the 20, 50 μmol/L ICA intervention group, the proliferation ability of SHED cells was significantly reduced. (2) In the 0.1, 1, 10 μmol/L ICA intervention group, the cell wound healing speed was faster. (3) In the 0.1, 1, 10 μmol/L ICA intervention group, the gene expression levels of DSPP and DMP1 were higher, and the protein expression levels of DSPP and DMP1 were higher in the 0.1 μmol/L ICA intervention group. (4) In the 1, 10 μmol/L ICA intervention group, the gene expression level of VEGFA was higher, and it had stronger tubular formation ability. (5) The expression of pro-inflammatory cytokines IL-1β, IL-6, TNF-α and IL-8 in the SHED cells in the 0.1, 1, 10 μmol/L ICA intervention group was significantly reduced.
CONCLUSION: Suitable concentrations of ICA can promote the proliferation, migration and odontoblast-like differentiation of SHED cells, enhance their angiogenic ability, and have a certain anti-inflammatory effect.