Complete genome sequence of Lacticaseibacillus rhamnosus B34-0-2 isolated from Panax ginseng in South Korea.

OBJECTIVES

Lacticaseibacillus rhamnosus is a widely studied probiotic species with notable functional diversity among strains. To expand the genomic resources available for this species and to support future comparative and probiotic-related studies, we sequenced and analyzed the complete genome of L. rhamnosus B34-0-2, a strain isolated from Panax ginseng, with a focus on its genomic features potentially associated with probiotic-related traits.

DATA DESCRIPTION: Genomic DNA was extracted and sequenced using a combination of PacBio long-read and Illumina short-read platforms. The assembled genome comprised 2,810 predicted coding sequences (CDSs), 59 tRNA genes, and 15 rRNA genes. Functional classification assigned 2,761 CDSs (98.25%) to Clusters of Orthologous Groups (COG) categories. Screening for bacteriocin-related genes identified three genomic regions with partial similarity; however, none satisfied the predefined criteria for confident bacteriocin annotation. No antibiotic resistance genes meeting the predefined identity and coverage thresholds were detected using the CARD database. Functional annotation revealed 37 reductase-related and 3 oxidase-related genes. Phylogenetic analysis based on the 16 S rRNA gene sequence indicated that the strain clusters with L. rhamnosus strain NGRI04. These genome data provide a genomic resource for future studies on functional characterization and probiotic-related properties.