Clinical and experimental dental research · 2026
Diagnostic Accuracy of Salivary Thiols Point-of-Care Test for Detecting Subgingival Dysbiosis in Adults: A Cross-Sectional Study.
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Khocht Ahmed, Rams Thomas E, Chen Tsute, Suzuki Jon B
Аннотация
OBJECTIVES: Many bacteria implicated in the pathogenesis of periodontitis produce thiol compounds containing sulfhydryl (-SH) groups. This cross-sectional study used a prototype chromogenic test strip to investigate the relationship between whole saliva -SH levels and subgingival bacterial species in subjects with and without periodontal disease.
MATERIALS AND METHODS: Ninety-six subjects were enrolled. Full-mouth comprehensive clinical periodontal evaluations were performed. Subjects were divided into 3 groups: periodontally healthy, gingivitis, and periodontitis. Subgingival plaque samples were collected and analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Subgingival dysbiosis severity was determined with a modified Subgingival Microbial Dysbiosis Index (mSMDI). Salivary thiol levels were determined semi-quantitatively with a chromogenic strip dipped in pooled saliva on the tongue surface.
RESULTS: Low and high strip scores were uniquely associated with contrasting groups of subgingival bacteria. Individuals with high strip scores harbored increased levels of P. gingivalis, P. intermedia, T. forsythia, P. nigrescens, E. nodatum, and T. denticola as well as higher mSMDI scores than individuals with low strip scores. Furthermore, strip scores showed high specificity (93%) in correctly identifying individuals with normobiotic subgingival microbiota.
CONCLUSIONS: Low salivary thiol levels are useful indicators of subgingival normobiosis.
MATERIALS AND METHODS: Ninety-six subjects were enrolled. Full-mouth comprehensive clinical periodontal evaluations were performed. Subjects were divided into 3 groups: periodontally healthy, gingivitis, and periodontitis. Subgingival plaque samples were collected and analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Subgingival dysbiosis severity was determined with a modified Subgingival Microbial Dysbiosis Index (mSMDI). Salivary thiol levels were determined semi-quantitatively with a chromogenic strip dipped in pooled saliva on the tongue surface.
RESULTS: Low and high strip scores were uniquely associated with contrasting groups of subgingival bacteria. Individuals with high strip scores harbored increased levels of P. gingivalis, P. intermedia, T. forsythia, P. nigrescens, E. nodatum, and T. denticola as well as higher mSMDI scores than individuals with low strip scores. Furthermore, strip scores showed high specificity (93%) in correctly identifying individuals with normobiotic subgingival microbiota.
CONCLUSIONS: Low salivary thiol levels are useful indicators of subgingival normobiosis.