MedElevator
Войти в исследования Frontiers in endocrinology · 2026
Integrative transcriptomics and metabolomics reveal Cry1-associated hepatic alterations and plasma essential fatty acids as biomarkers of residual circadian disruption post-sepsis.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhao Junzhou, Huang Tianxiao, Wang Li, Shi Yun, Zou Wanni, Chen Yunxuan, Su Qisheng, Qiao Linlan, Wu Zheng, Lv Yi, Zhang Yuanyuan, Wu Rongqian
Аннотация
BACKGROUND: Sepsis disrupts circadian rhythm, leaving lasting molecular traces and circadian insensitivity to jet lag even after acute phase recovery. However, the core mechanism and biomarkers of residual circadian dysfunction post-sepsis remain unclear, which hinders targeted interventions.
METHODS: Male mice were subjected to non-lethal LPS injection, and after 7 days of recovery, liver transcriptomics and plasma metabolomics were integrated. Behavioral, physiological, and molecular analyses were performed, with statistical tests including DESeq2, Student's t-test, MetaCycle, and ROC curve analysis.
RESULTS: Significant transcriptomic alterations were observed, most prominently at Zeitgeber time 21. Linoleic acid (LA) and alpha-linolenic acid (ALA) were identified as sampling-time-independent candidate biomarkers associated with post-sepsis circadian disruption. Exogenous supplementation of these two fatty acids shortened male mice's rhythm re-entrainment period.
CONCLUSIONS: This study identifies a hypothesized Cry1-essential fatty acid axis potentially contributing to post-sepsis circadian dysfunction based on multi-omics associations, providing novel biomarkers and a metabolic intervention strategy for detecting and treating circadian rhythm disorders in sepsis survivors.
METHODS: Male mice were subjected to non-lethal LPS injection, and after 7 days of recovery, liver transcriptomics and plasma metabolomics were integrated. Behavioral, physiological, and molecular analyses were performed, with statistical tests including DESeq2, Student's t-test, MetaCycle, and ROC curve analysis.
RESULTS: Significant transcriptomic alterations were observed, most prominently at Zeitgeber time 21. Linoleic acid (LA) and alpha-linolenic acid (ALA) were identified as sampling-time-independent candidate biomarkers associated with post-sepsis circadian disruption. Exogenous supplementation of these two fatty acids shortened male mice's rhythm re-entrainment period.
CONCLUSIONS: This study identifies a hypothesized Cry1-essential fatty acid axis potentially contributing to post-sepsis circadian dysfunction based on multi-omics associations, providing novel biomarkers and a metabolic intervention strategy for detecting and treating circadian rhythm disorders in sepsis survivors.