Frontiers in neurology · 2026
The impact of stroke on intramuscular connective tissue in mice.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhao Xiaoxiao, Morri Giulio, D'Urso Matteo, Fede Caterina, Petrelli Lucia, Vignozzi Livia, Sun Yunfeng, Clair Claudia, Deidda Gabriele, Raghavan Preeti, Allegra Manuela, Stecco Carla
Аннотация
BACKGROUND: Previous studies about post-stroke peripheral structural remodeling have been focused on muscle fiber alterations, while extracellular matrix (ECM) remodeling in musculoskeletal tissues remains unclear. This study examines changes in extracellular matrix (ECM), specifically hyaluronan (HA) and collagen, in forelimb muscles following distal middle cerebral artery occlusion (dMCAO) in mice.
METHODS: Twenty-two 8-week-old C57BL/6 mice (10 male, 12 female) were randomly assigned to the control group (n = 10; 4 male, 6 female) or the experimental group (n = 12; 6 male, 6 female). 4 weeks post-stroke, forelimb muscle samples were collected and analyzed for HA and collagen distribution and concentrations.
RESULTS: HA concentrations were significantly elevated in both the left (stroke affected) forelimb muscles (63.74 ± 11.80 μg/g), and right (unaffected) forelimb muscles (64.16 ± 13.52 μg/g) of stroke mice compared to the controls (left: 50.07 ± 8.84 μg/g, p = 0.022; and right: 49.36 ± 12.22 μg/g, p = 0.015). No significant difference in HA levels was observed between the affected and unaffected sides of stroke mice (p = 0.905). Collagen concentrations did not differ significantly among groups (control left: 8.62 ± 2.91 μg/g; control right: 8.77 ± 1.35 μg/g; stroke-affected: 8.24 ± 2.11 μg/g; stroke-unaffected: 8.28 ± 2.45 μg/g).
CONCLUSION: The results revealed a systemic increase in HA concentration in the stroke group, while collagen levels remained unchanged. These findings provide biochemical evidence of early alterations in extracellular matrix composition after stroke.
METHODS: Twenty-two 8-week-old C57BL/6 mice (10 male, 12 female) were randomly assigned to the control group (n = 10; 4 male, 6 female) or the experimental group (n = 12; 6 male, 6 female). 4 weeks post-stroke, forelimb muscle samples were collected and analyzed for HA and collagen distribution and concentrations.
RESULTS: HA concentrations were significantly elevated in both the left (stroke affected) forelimb muscles (63.74 ± 11.80 μg/g), and right (unaffected) forelimb muscles (64.16 ± 13.52 μg/g) of stroke mice compared to the controls (left: 50.07 ± 8.84 μg/g, p = 0.022; and right: 49.36 ± 12.22 μg/g, p = 0.015). No significant difference in HA levels was observed between the affected and unaffected sides of stroke mice (p = 0.905). Collagen concentrations did not differ significantly among groups (control left: 8.62 ± 2.91 μg/g; control right: 8.77 ± 1.35 μg/g; stroke-affected: 8.24 ± 2.11 μg/g; stroke-unaffected: 8.28 ± 2.45 μg/g).
CONCLUSION: The results revealed a systemic increase in HA concentration in the stroke group, while collagen levels remained unchanged. These findings provide biochemical evidence of early alterations in extracellular matrix composition after stroke.