The role of cortisol in the acute immune regulation of channel catfish (Ictalurus punctatus) spleen stimulated by Aeromonas hydrophila.

Channel catfish (Ictalurus punctatus), a significant aquaculture species, occupies a prominent position in the aquaculture industry due to its rapid growth, excellent adaptability, and economic value; however, the hemorrhagic disease caused by Aeromonas hydrophila has had a substantial impact on its cultivation. Researches have indicated that cortisol, the main stress hormone, is essential for regulating immune responses. Therefore, in this study, the immune regulatory effects of cortisol on the spleen tissue under Aeromonas hydrophila stimulation were analyzed. Through transcriptomic (RNA-seq) analysis, we identified 167 differentially expressed genes (DEGs) regulated by cortisol. The KEGG enrichment analysis indicated that the DEGs were predominantly associated with various biological pathways, including antigen processing and presentation, bladder cancer, autophagy in animals, lipid metabolism, and atherosclerosis. Protein-protein interaction network analysis further indicated that these DEGs participate in key signaling pathways, including HIF, JAK-STAT, and NF-KB. Our findings demonstrate that cortisol exerts an immunoregulatory effect by modulating these key signaling pathways in the spleen tissue infected with Aeromonas hydrophila, which is of significant importance for understanding the mechanism of cortisol in fish immune responses.