Pest management science · 2025
RNAi of 20S proteasome β subunit 3 inhibits midgut homeostasis and the survival of the migratory locust, Locusta migratoria.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yang Yong, Wang Mingjun, Guo Wenhui, Zhu Qingyao, Zhang Daochuan, Zhang Xiaohong
Аннотация
BACKGROUND: Tissue homeostasis is essential for both morphogenesis and maintaining function. In insects, intestinal homeostasis is critical for growth and development. The midgut is key in food digestion, nutrient absorption, and immune response. Locusta migratoria is among the most damaging agricultural pests worldwide. Identifying a suitable target gene is important for RNA interference (RNAi)-based pest control.
RESULTS: In this study, RNAi-mediated knockdown of the 20S proteasome β subunit 3 of L. migratoria (LmProsβ3) caused atrophy of the midgut and gastric caecum in fourth- and fifth-instar nymphs as well as adult locusts. This deficiency also led to a decrease in body length and weight, eventually resulting in mortality. Histological analysis using haematoxylin and eosin (H&E) staining and transmission electron microscopy (TEM) showed that LmProsβ3 knockdown damaged columnar epithelial cells and regenerative cells, with the nucleus disappearing due to cell cycle inhibition. Additionally, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the dsLmProsβ3 group were higher than those in the double-stranded green fluorescent protein (dsGFP) group. Moreover, the activities of alkaline phosphatase (AKP) and acid phosphatase (ACP) in the double-stranded LmProsβ3 (dsLmProsβ3) group were reduced.
CONCLUSION: LmProsβ3 is essential for midgut homeostasis and survival, influencing the growth and development of locusts. These findings help clarify the functions of LmProsβ3 in maintaining midgut health. LmProsβ3 could be a potential target gene for RNAi-based locust control. © 2025 Society of Chemical Industry.
RESULTS: In this study, RNAi-mediated knockdown of the 20S proteasome β subunit 3 of L. migratoria (LmProsβ3) caused atrophy of the midgut and gastric caecum in fourth- and fifth-instar nymphs as well as adult locusts. This deficiency also led to a decrease in body length and weight, eventually resulting in mortality. Histological analysis using haematoxylin and eosin (H&E) staining and transmission electron microscopy (TEM) showed that LmProsβ3 knockdown damaged columnar epithelial cells and regenerative cells, with the nucleus disappearing due to cell cycle inhibition. Additionally, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the dsLmProsβ3 group were higher than those in the double-stranded green fluorescent protein (dsGFP) group. Moreover, the activities of alkaline phosphatase (AKP) and acid phosphatase (ACP) in the double-stranded LmProsβ3 (dsLmProsβ3) group were reduced.
CONCLUSION: LmProsβ3 is essential for midgut homeostasis and survival, influencing the growth and development of locusts. These findings help clarify the functions of LmProsβ3 in maintaining midgut health. LmProsβ3 could be a potential target gene for RNAi-based locust control. © 2025 Society of Chemical Industry.