Metal Ion-Peptide-Intermediated Ligand Transfer for Serum Detection of the Omicron Variant of SARS-Cov-2 in Echinococcosis Patients.

A method is developed to electrochemically induce target-specific covalent capturing of the spike protein of SARS-Cov-2, forming a covalent peptide-protein complex fit for working with such complicated clinical samples. Specifically, peptide-coordinated copper ions can be electrochemically controlled to induce cross-linkage between certain amino acids on the peptide probe and the target protein. Therefore, target specificity can be tuned electrochemically, realizing highly specific targeting of the omicron S protein or broader specificity toward all variants of the virus. Using this method, with electrochemically catalyzed generation of signal-enhancing molecules, the sensitivity and covalent detection allow their application in both serum and fecal samples. These results may point to their possible use in screening new variants of the virus in the near future.