Neuron · 2022
Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients.
СтатьяНазвание и аннотация: Перевод в очереди
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Khan Mona, Clijsters Marnick, Choi Sumin, Backaert Wout, Claerhout Michiel, Couvreur Floor, Van Breda Laure, Bourgeois Florence, Speleman Kato, Klein Sam, Van Laethem Johan, Verstappen Gill, Dereli Ayse Sumeyra, Yoo Seung-Jun, Zhou Hai, Dan Do Thuc Nguyen, Jochmans Dirk, Laenen Lies, Debaveye Yves, De Munter Paul, Gunst Jan, Jorissen Mark, Lagrou Katrien, Meersseman Philippe, Neyts Johan, Thal Dietmar Rudolf, Topsakal Vedat, Vandenbriele Christophe, Wauters Joost, Mombaerts Peter, Van Gerven Laura
Аннотация
Can SARS-CoV-2 hitchhike on the olfactory projection and take a direct and short route from the nose into the brain? We reasoned that the neurotropic or neuroinvasive capacity of the virus, if it exists, should be most easily detectable in individuals who died in an acute phase of the infection. Here, we applied a postmortem bedside surgical procedure for the rapid procurement of tissue, blood, and cerebrospinal fluid samples from deceased COVID-19 patients infected with the Delta, Omicron BA.1, or Omicron BA.2 variants. Confocal imaging of sections stained with fluorescence RNAscope and immunohistochemistry afforded the light-microscopic visualization of extracellular SARS-CoV-2 virions in tissues. We failed to find evidence for viral invasion of the parenchyma of the olfactory bulb and the frontal lobe of the brain. Instead, we identified anatomical barriers at vulnerable interfaces, exemplified by perineurial olfactory nerve fibroblasts enwrapping olfactory axon fascicles in the lamina propria of the olfactory mucosa.