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Gut microbiota remodeling in HBB-mutant cynomolgus monkeys reveals blood-gut axis disruption associated with β-thalassemia-related gastrointestinal dysfunction.

Gastrointestinal symptoms frequently accompany anemia caused by HBB mutations, such as β-thalassemia; however, the mechanisms linking disord … anoic acid (HMDB0254633) emerging as a discriminative metabolite between WT and HBB-mutant animals. Multiomics integration indicated that HBB mutation reshaped microbiota-metabolite interactions and may thereby affect host metabolism and immune responses. To examine the functional relevance of this metabolite, 3-oxooctadecanoic acid was administered to C57BL …

Machine Learning in Nonhuman Primate Models of Infectious Diseases: Current Applications and Future Perspectives.

The amount of biologic data produced by biomedical research has increased significantly in both volume and complexity in recent years. Advances in computational power have increasingly enabled the use of machine learning (ML) to analyze and predict patterns from large-scale, complex biologic datasets. In nonhuman primate (NHP) infectious disease models, such high-dimensional datasets containing a large number of features are often obtained by next-generation sequencing-based multiomics and immunologic analyses. As a result, ML is particularly valuable for effective analysis and predictive mode…

Integrative Omics Analysis Reveals a CD206+ Macrophage/Dendritic Cell-CD155-TIGIT+ Regulatory T Cell Axis as a Protective Immune Checkpoint Pathway in Atherosclerosis Progression.

RNA sequencing (RNA-seq) analysis revealed transcriptional features indicative of sustained immunosuppression within regulatory T cells (Tregs) isolated from atherosclerotic tissues, highlighting the critical contribution of immune checkpoint (ICP) receptor-ligand interactions to atherosclerosis progression. To comprehensively characterize the roles of ICP signaling in this context, we integrated transcriptomic profiling, quantitative RT-PCR, and flow cytometry analysis, yielding several key findings: (1) Multiomics integration revealed global transcriptomic alterations in ICP receptors and li…

Breast cancer immunotherapy: mechanisms of immune evasion, biomarkers, and emerging therapeutic strategies.

Breast cancer remains the most prevalent malignancy worldwide and a major cause of cancer-related mortality, representing a heterogeneous an … Finally, we explore future directions emphasizing personalized, multimodal immunotherapy guided by multiomics and spatial profiling, integrated with artificial intelligence. Finally, we synthesize a framework for advancing immunotherapy in breast cancer and identify key areas requiring further translational and clinical investigation.

Shaoyao Gancao decoction ameliorates vascular dementia by modulating neuroinflammation and oxidative stress pathways.

BACKGROUND: Vascular dementia (VaD) is a prevalent neurodegenerative disorder for which effective disease-modifying therapies are currently … d significantly ameliorate cognitive deficits and alleviate pathological damage in a VaD rat model. Multiomics integration analysis nominated XDH and EIF2AK2 as candidate targets potentially associated with the regulation of neuroinflammation and oxidative stress through the TLR4/NF-κB/NLRP3 signaling axis. Both molecular dynamics simulations and experimenta …

A Multidimensional Strategy of Flavor Evaluation Linking Yeast-Driven Chemical Networks to Sensory Attributes of Cherry Wine.

This study systematically investigated the differential impacts of five commercial yeast strains on the chemical fingerprint and sensory attributes of cherry wine through integrating multiomics flavor analysis and sensory evaluation. During fermentation, total sugar content decreased from 220 g/L to 4.8-5.3 g/L, resulting in an ethanol concentration of around 11.0% (v/v). Cherry wine fermented with SIHA7 yeast exhibited the highest antioxidant capacity, whereas 71B yeast was particularly effective in reducing malic acid levels. This strain also showed an association with higher lactic acid con…

Genetic background influences developmental airway smooth muscle program and susceptibility to airway hyperresponsiveness in mice.

Airway structural remodeling and hyperresponsiveness (AHR), hallmarks of asthma, are influenced by genetic variations and adverse exposures. … d (RA)-disrupted intrauterine environment in vivo by maternal BMS493 administration. Single-nucleus multiomics identified a mesenchymal cell population overactivating TGF-β targets in response to BMS493 selectively in A/J lungs. These cells, localized to sites of airway SM initiation and p-SMAD2- and -3, exhibited robust BMS493-mediated upregulation of SMAD2 …

Noncoding RNA networks in endodontic regeneration: Current evidence and future directions.

AIM: This narrative review provides a consolidated existing literature on regulatory functions of noncoding RNAs (ncRNAs), including microRN … erative endodontics that connects molecular biology into clinical translational. The combination of multiomics analyses along with biomaterial-based delivery systems can lead to precision, mechanism-driven regenerative therapies and may also transform future endodontic treatment modalities.

SRSF1/Mcl-1 Axis Drives Apoptosis Evasion and Shapes the Immune Microenvironment to Promote Gastric Cancer Progression.

Gastric cancer (GC) is one of the malignancies with the highest incidence and mortality worldwide. Evasion of apoptosis is a hallmark of can … ed in GC tissues and cell lines, correlating with poor prognosis in patients. Through comprehensive multiomics analysis, we first revealed that SRSF1-positive malignant epithelial cells possess a unique coexpression network and exhibit significantly enhanced interactions with fibroblasts, reshaping the tumor microenvironment (TME). Subsequently, functional a …

Patient-derived organoids in gynaecological cancers: emerging tools for therapy and disease modelling.

Patient-derived organoids (PDOs) have emerged as physiologically relevant three-dimensional (3D) culture systems that closely mimic the mole … can be further improved by incorporating organ-on-a-chip platforms, CRISPR/Cas9 genome editing, and multiomics profiling. These biobanking programmes and microfluidic advances will permit longitudinal analyses as well as high-throughput screenings for targeted therapies against inhibitors of PARP, PI3K-AKT, and hormonal pathways. Although several technical c …

Network dysregulation in depression: A synthesis of HPA Axis, BDNF signaling, and neurotransmitter interactions across multidimensional systems.

Depression is a complex mental disorder threatening global public health. Its pathophysiological mechanisms involve multidimensional dysfunc … out the limitations of current research and prospects the precision research direction integrating multiomics and neuroimaging, reconceptualizing depression as a network disorder centered on the abnormality of the BDNF/CREB signaling hub, so as to provide theoretical support for elucidating the pathological mechanism of depression and developing precision d …

Ферроптоз по нейродегенеративным болезням: Регулирование железной диссы, сигнатуры GPX4/FSP1 и новые стратегии защиты нейропов.

ВВЕДЕНИЕ: Нейродегенеративные заболевания (НДД), включая болезнь Альцгеймера (AD), болезнь Паркинсона (PD), амиотрофический боковой склероз (ALS), болезнь Хантингтона (HD) и множественный склероз (MS), все чаще ассоциируются с ферропотозом, регулируемой железозависимой формой смерти клеток, характеризующейся чрезмерным накоплением липидовых пероксидов. МЕТОДЫ: В настоящем обзоре анализируются молекулярные основания ферроптоза, при этом основное внимание уделяется регулированию дизакций в гомеостазе железа, повышению уязвимости полиненасыщенных жировых кислот в нейрональных мембранах, а также п

Совпадение в аннотации оригинала
INTRODUCTION: Neurodegenerative Diseases (NDDs), including Alzheimer's Disease (AD), Parkinson's Disease (PD), Amyotrophic Lateral Sclerosis … idative and metal-induced neurotoxicity in neurodegenerative disorders. Future research integrating multiomics profiling, longitudinal clinical data, and advanced computational modelling is essential to validate the efficacy of ferroptosis-targeted interventions and facilitate their successful translation into precision neurotherapeutics.

Update in the molecular mechanism and biomarkers of diabetic retinopathy.

Diabetic retinopathy (DR) is a serious complication of diabetes caused by long-term hyperglycemia that leads to microvascular and neuronal d … ges, including the activation of glial cells, neuronal dysfunction, and cell death. Research on the multiomics molecular markers of DR has revealed complex mechanisms at the genetic, epigenetic, and transcriptional levels. Genome-wide association studies (GWASs) have identified multiple genetic loci associated with DR that are involved in metabolic and infla …

Integrated analysis of scRNA-seq and bulk RNA-seq data identifies BHLHE40 as a key gene in pancreatic cancer progression and gemcitabine resistance.

OBJECTIVE: Pancreatic cancer is characterized by its high mortality rate and short survival periods, and novel therapeutic targets and tailor personalized strategies are urgently needed. In this study, we aim to investigate the molecular mechanisms underlying pancreatic ductal adenocarcinoma (PDAC) progression and chemoresistance, with a focus on identifying novel therapeutic targets. METHODS: Multiomics approaches were integrated to identify novel actionable targets for PDAC. Public datasets such as TCGA and GEO were utilized to investigate the relationship between gene expression and clinic…

CD2AP is a disulfidptosis modulator and prognostic biomarker in hepatocellular carcinoma.

CD2-associated protein (CD2AP) is an adapter protein that stabilizes the actin cytoskeleton and is closely linked to disulfidptosis. However, its functional role in hepatocellular carcinoma (HCC) progression and disulfidptosis remains undefined. Herein, pan-cancer multiomics analyses were performed on 33 malignancies from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) cohorts to evaluate CD2AP expression patterns, prognostic significance, and interactions with the tumor immune microenvironment. Clinical validation was performed using 70 HCC specimens, with qRT-PCR analysi…

How "Omics" Studies Contribute to a Better Understanding of Fuchs' Endothelial Corneal Dystrophy.

Fuchs' endothelial corneal dystrophy (FECD) is a progressive eye disease characterized by accelerated loss of endothelial cells and the deve … e most significant biological pathways related to extracellular-matrix organization. In the future, multiomics study approaches are needed to increase the sample size and statistical power to identify strong candidate genes for functional studies on animal models and cell lines for better understanding FECD.

Integrative genomics and functional immunology reveal Clostridia species as modulators of neuroinflammation in amyotrophic lateral sclerosis.

OBJECTIVE: This multiomics study investigated causal relationships between the gut microbiota (GM), immune dysregulation, and amyotrophic lateral sclerosis (ALS) pathogenesis using Mendelian randomization (MR) with experimental validation. MATERIALS: Analyses incorporated genome-wide data from 87,347 participants (GM: n = 7738; ALS: 20,806 patients, 59,804 controls; immune phenotypes: n = 3757), transcriptomic data from 71 subjects (56 ALS patients, 15 controls), and experimental validation in matched cohorts (n = 6 subjects per group). METHODS: Two-sample bidirectional MR and mediation anal…

Multimodal Mass Spectrometry Imaging with a Metal-Based Alignment Tool Enables Targeted Single-Cell Boronophenylalanine Uptake and Metabolic Profiling in Glioblastoma Tumors.

Mass spectrometry imaging (MSI) is central to spatial multiomics; however, integrating multimodal MSI techniques and achieving accurate image coregistration remain major challenges. Here, we present an integrated multimodal MSI platform empowered by a novel metal-based alignment tool (MAT) for accurate coregistration across multimodal MSI. This platform combines laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOF-MS) for single-cell elemental and isotope-tagged protein imaging with desorption electrospray ionization mass spectrometry (DESI-MS) for spatial met…

Lipidomics reveals different therapeutic potential for natural and synthetic vitamin D formulations in hepatocyte lipotoxicity.

Natural sources of vitamin D (VD), have been proposed to represent an alternative to synthetic vitamin D in nutritional interventions, also … ay (transcriptomics) data available from previous studies on this experimental model; the resulting multiomics data identified lipid metabolism nodes specific for the multimolecular mechanisms of the two formulations which may deserve further pre-clinical investigation in the treatment of hepatocyte lipotoxicity.

Identification of CSRP1 as novel biomarker for hormone-sensitive prostate cancer by the combination of clinical and functional research.

Prostate cancer (PCa) ranks as the second most common malignancy and the fifth leading cause of cancer-related deaths among men. A critical challenge lies in accurately identifying those patients at high risk for transitioning rapidly from hormone-sensitive PCa (HSPC) to lethal castration-resistant PCa (CRPC). In our study, we employed a multiomics approach involving bioinformatics analysis on datasets GSE2443 and GSE35988, along with proteomics studies, to discover that cysteine- and glycine-rich protein 1 (CSRP1) expression significantly impacts the progression of HSPC. This hypothesis was s…