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Investigations of bone turnover in renal osteopathy.

The renal bone disease which develops in chronic renal failure (CRF) is not an uniform disorder. Histomorphometry is accepted to be the best method for characterising the state of disease. The purpose of this study was to evaluate the suitability of pyridinium crosslinks in serum and urine as indicators of bone degradation processes. Patients with CRF had significantly higher Pyridinoline (Pyd) and Deoxypyridinoline (Dpyd) levels in serum and urine compared to normal controls except the urinary excretion in the subgroup of glomerulonephritis. A correlation was found between the serum levels of

Inflammation hinders anabolic potential of nutritional support in cancer: findings from the Protein Recommendation to Increase Muscle (PRIMe) trial.

BACKGROUND: Our recent Protein Recommendation to Increase Muscle (PRIMe) trial suggested that increasing daily protein intake via nutrition … was explained by baseline concentrations of interleukin (IL)-6, insulin, and specific amino acids (hydroxyproline, aspartate, taurine) and protein intake changes (P = 0.021). By 12 wk, IL-6 and methionine explained only 32% (P = 0.030). Tumor stage and concurrent chemotherapy type did not play a role. Positive LST change after 6 wk occurred at daily protein inc …

MSC Origin and Biomechanical Conditioning Determine ECM Maturation in Tissue-Engineered Matrix.

Background: The extracellular matrix (ECM) plays a central role in the mechanical strength and functional integration of tissue-engineered m … (Picrosirius Red), alongside biochemical assays that quantified DNA, glycosaminoglycan (GAGs), and hydroxyproline (HYP). Results: Hydrodynamically stimulated culture consistently improved ECM deposition across all groups. TEMs exposed to hydrodynamic stimulation (hydrodynamic conditions) were thicker, more uniformly filled, and exhibited increased collagen depo …

Efficacy and Safety of Centella asiatica in Wound Healing: A Systematic Review and Meta-Analysis.

ETHNOPHARMACOLOGICAL RELEVANCE: Centella asiatica (L.) Urb. is a medicinal herb belonging to the family Apiaceae. It is commonly known as Br … ization period, wound contraction efficiency, and wet and dry tissue weights), biochemical markers (hydroxyproline content, collagen deposition, and tissue protein levels), and histopathological features (neovascularization, reduced inflammation, and increased fibroblast proliferation). Furthermore, three studies evaluating acute dermal toxicity according to OEC …

Comparison of predicted C-peptide structures of human and venom insulin G1: A putative γ-glutamyl carboxylase recognition motif in venom insulin.

The connecting peptide (C-peptide) of human insulin has been implicated in native disulfide bond formation and diverse biological functions; … e features, including posttranslational modifications such as γ-carboxyglutamic acid (Gla), 4-trans-hydroxyproline, and C-terminal amidation, as well as a monomeric solution state and rapid activation of the human insulin receptor. Using AlphaFold 3-predicted proinsulin structures, this study investigates hydrophobicity profiles, disulfide conformations, and oxi …

miR149-5p: A hepatocyte EXOmotif sequence containing microRNA that attenuates liver fibrosis by specifically targeting PDGFRB in HSCs.

INTRODUCTION: Liver fibrosis is a progressive pathological process driven by liver injury, including viral hepatitis and metabolic dysfuncti … enuated BDL- and TAA-induced liver fibrosis, as evidenced by reduced collagen deposition, decreased hydroxyproline content, and downregulation of HSC activation markers. CONCLUSION: The PU.1 (hepatocyte)-miR-149-5p (exosome)-PDGFRB (HSC) axis represents a key regulatory pathway in liver fibrosis, wherein PU.1 represses miR-149-5p transcription in hepatocytes, l …

Impaired organic and mineral extracellular matrix composition in early-onset osteoporosis.

Early-onset osteoporosis (EOOP) is a rare form of primary osteoporosis defined by major skeletal fractures or low BMD that occurs in early a … ity density (p < .001); cortical thickness was lower in EOOP BBs (p < .01). Also, GAG/Amide III and hydroxyproline/proline ratios as well as accumulation of AGEs were greater in EOOP than controls (all p < .0001). Moreover, tissue mineralization was lower in EOOP than controls, as shown by v1PO4/CH2 ratio, mineral maturity crystallinity and crystal size index (a …

Diosgenin alleviates hepatic fibrosis via PI3K/Akt/mTOR mediated fatty acid metabolic reprogramming and NF-κB activation.

ETHNOPHARMACOLOGICAL RELEVANCE: Dioscorea oppositifolia L., also called Shan Yao in traditional Chinese medicine, is widely used to treat di … damage, collagen deposition and α-SMA expression, while reducing serum levels of ALT, AST, ALP, and hydroxyproline. In vitro, DIO suppressed TGF-β1-induced proliferation, activation, and fibrogenic gene expression in LX2 cells. Network pharmacology and molecular docking identified mTOR and PIK3CA as direct binding targets of DIO, which was further validated by c …

Сравнительное исследование моделей мыши острого обострения фиброза легких.

Этиология и основополагающие механизмы острого обострения идиопатического фиброза легких (AE-IPF) остаются недостаточно понятными. Несмотря на то, что для изучения AE-IPF было разработано несколько моделей для животных, систематическая оценка и сопоставление этих моделей еще не были представлены. В настоящем исследовании PF был индуцирован у мышей одним внутритрахеальным управлением блюмицином (BLM). На 14-й день после первоначального вызова BLM AE-PF в мышах была вызвана внутритрахеальной репробацией аденовальных векторов (ADV), липополизакаридом (LPS) или второй дозой BLM. Микрочестная вычис

Совпадение в аннотации оригинала
The etiology and underlying mechanisms of acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) remain poorly understood. Although se … s well as more extensive lung injury on CT. Histopathological scores for inflammation and fibrosis, hydroxyproline content and expression levels of fibrotic markers (fibronectin, collagen I, α-smooth muscle actin and MMP7) were significantly elevated in the AE-PF groups. Inflammatory cytokines (IL-6, IL-1β and TNF-α) were markedly increased in both serum and BAL …

Fuzheng Huayu formula attenuates ductular reaction and liver fibrosis potentially through suppressing Gli1 pathway.

ETHNOPHARMACOLOGICAL RELEVANCE: Fuzheng Huayu formula (FZHY) is clinically used for liver fibrosis treatment, but its pharmacological mechan … Y obviously ameliorated liver fibrosis, evidenced by reduced collagen deposition, decreased hepatic hydroxyproline content, and less inflammatory cell infiltration. FZHY reduced the expression of Epcam, CK19, and CK7. Double-immunofluorescence revealed an increased number of CK19+ and OV6+ cells in the CCl4/2-AAF rats, which decreased after FZHY treatment. Mecha …

The Simultaneous Deletion of pH-Sensing Receptors GPR4 and OGR1 (GPR68) Ameliorates Colitis with Additive Effects on Multiple Parameters of Inflammation.

G protein-coupled receptors (GPRs), including pro-inflammatory GPR4 and ovarian cancer GPR1 (OGR1/GPR68), are involved in the pH sensing of … a decrease in the number of macrophages in the colon, as compared to single deletions. Importantly, hydroxyproline levels were decreased close to baseline in Il10-/- × Gpr4-/- × Ogr1-/- mice. Our findings demonstrate that the simultaneous loss of GRP4 and OGR1 functions exerts an additive effect on multiple parameters associated with colonic inflammation. These …

Transient angiotensin-converting enzyme inhibition confers sex-specific protection against angiotensin II-induced cardiac remodeling.

Hypertension increases the prevalence of heart failure to a greater extent in women than men. The fibrotic remodeling of the left ventricle … 3/SMAD3, periostin, and lysyl oxidase (LOX)] and pro-oxidative proteins (NOX2 and NOX4), as well as hydroxyproline (HYP) content similarly in both sexes. Interestingly, a positive correlation between angiotensin II type 1 (AT1) receptor gene expression and Col1a1 in Ang II-treated males is absent in the female SHRs. The observed sex differences in the protection …

LncRNA Meg3 Aggravates Renal Fibrosis Caused by Unilateral Ureteral Obstruction in Rats by Activating the Hedgehog Pathway.

BACKGROUND: The hedgehog signaling pathway exerts vital functions in regulating epithelial-to-mesenchymal transition (EMT) in renal intersti … ts and renal tissue of rats; kits for testing levels of blood urea nitrogen (BUN), creatinine (Cr), hydroxyproline (HYP), and 24-hour urine protein (24-up) in rats of each group; hematoxylin and eosin (HE) staining and Masson staining for observing kidney tissue and renal fibrosis level in rats; western blot for measuring levels of collagen type III (Col III), α …

Long-lasting, biochemically modified mRNA, and its frameshifted recombinant spike proteins in human tissues and circulation after COVID-19 vaccination.

According to the CDC, both Pfizer and Moderna COVID-19 vaccines contain nucleoside-modified messenger RNA (mRNA) encoding the viral spike gl … n in circulation with organ-related functional damage even in asymptomatic individuals. Proline and hydroxyproline residues emerge as prominent deuterium (heavy hydrogen) binding sites in structural proteins with robust isotopic stability that resists not only enzymatic breakdown, but virtually all (non)-enzymatic cleavage mechanisms known in chemistry.

Opposite effects of systemic and local conditional CD11c+ myeloid cell depletion during bleomycin-induced inflammation and fibrosis in mice.

RATIONALE: Elevated levels of CD11c+ myeloid cells are observed in various pulmonary disorders, including Idiopathic Pulmonary Fibrosis (IPF … Increased anti-inflammatory cytokine IL-10 and CXCL9 levels were observed, resulting in lower lung hydroxyproline content and Ashcroft fibrosis score. Conversely, local depletion of CD11c+ cells increased mortality by acute leukocyte influx (predominantly neutrophils, DCs, and MΦ in BAL) correlated to IL-1β, with lung hyper-inflammation and early fibrosis devel …

Collagen architecture and biomechanics of gracilis and adductor longus muscles from children with cerebral palsy.

Cerebral palsy (CP) describes some upper motoneuron disorders due to non-progressive disturbances occurring in the developing brain that cau … reconstruction surgery with hamstring autograft. Muscle mechanical testing, two-photon imaging and hydroxyproline assay were performed on biopsies. Corresponding data were compared to radiographic hip displacement in CP adductors (CPA), gait kinematics in CP hamstrings (CPH), and joint range of motion in CPA and CPH. We found at matched sarcomere lengths muscle …