Результаты поиска

mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge.

The recent outbreaks of mpox have raised concerns over the need for effective vaccines. However, the current approved vaccines have either been associated with safety concerns or are in limited supply. mRNA vaccines, which have shown high efficacy and safety against SARS-CoV-2 infection, are a promising alternative. In this study, three mRNA vaccines are developed that encode monkeypox virus (MPXV) proteins A35R and M1R, including A35R extracellular domain -M1R fusions (VGPox 1 and VGPox 2) and a mixture of encapsulated full-length mRNAs for A35R and M1R (VGPox 3). All three vaccines induce ea…

Gene expression profiling of monkeypox virus-infected cells reveals novel interfaces for host-virus interactions.

Monkeypox virus (MPV) is a zoonotic Orthopoxvirus and a potential biothreat agent that causes human disease with varying morbidity and mortality. Members of the Orthopoxvirus genus have been shown to suppress antiviral cell defenses, exploit host cell machinery, and delay infection-induced cell death. However, a comprehensive study of all host genes and virus-targeted host networks during infection is lacking. To better understand viral strategies adopted in manipulating routine host biology on global scale, we investigated the effect of MPV infection on Macaca mulatta kidney epithelial cells …

Отклонение временных тенденций передачи вируса обезболивающих обезьян в Бурунди.

Используя данные по мпоксу из Бурунди в период с августа 2024 года по апрель 2025 года, мы откалибровали математическую модель для количественной оценки временных тенденций передачи вируса обезьяноксика. Результаты, полученные с помощью модели, свидетельствуют о снижении общей тенденции передачи. Согласно оценкам, дети в возрасте 0 - 4 и 5 - 9 лет подвергаются более высокому риску инфицирования по сравнению с пожилыми возрастными группами, в то время как сексуальные контакты, как представляется, составляют до 50% общей передачи инфекции.

Совпадение в аннотации оригинала
Utilizing mpox case data from Burundi between August 2024 and April 2025, we calibrated a mathematical model to quantify the temporal trends of clade Ib monkeypox virus transmission. The model outputs indicated a declining overall transmission trend. Children aged 0-4 and 5-9 years were estimated to be at higher risk of infection compared to older age groups…

3-Hydroxypyrimidine-2,4-dione derivatives as monkeypox virus resolvase (Mpr) inhibitors.

Holliday junction resolvase plays an important role in poxvirus genome replication and viral maturation. Recently, we developed the first in vitro assay measuring the activity of orthopoxvirus resolvases, particularly monkeypox virus resolvase (Mpr). Screening of our in-house compound library identified metal-chelating chemotypes as resolvase inhibitors, including 3-hydroxypyrimidine-2,4-dione (HPD). Herein we report the synthesis and Mpr-based structure-activity relationship (SAR) of 37 new HPD analogs. The SAR focused on the effects of various arylamino groups at C-6 and N-1 methyl substitut…

Транскриптомный и протеомический анализ вирусов обезьяны A5L-Expressing HEK293T.

Mopkeypox (MPOX) — зоонотическая вирусная болезнь, вызванная вирусом Monkeypox (MPXV), ставшая самой серьезной угрозой для здоровья населения в ортопоксирусном роде после ликвидации вируса Variola (VARV). Несмотря на то, что MPXV привлекла большое внимание, мало что известно о его клинических проявлениях у людей. В этом исследовании для изучения транскрипционной и метаболической реакции клеток HEK293T на белок MPXV A5L использовался высокопроизводительный подход последовательности RNA (RNA-seq) и жидкой хроматографии-тандем масс-спектрометрии (LC-MS/MS). Анализ RNA-seq выявил в общей сложности

Совпадение в аннотации оригинала
Monkeypox (MPOX) is a zoonotic viral disease caused by the Monkeypox virus (MPXV), which has become the most significant public health threat within the Orthopoxvirus genus since the eradication of the Variola virus (VARV). Despite the extensive attention MPXV has garnered, little is known about its clinical manifestations in humans. In this study, a high-th…

Chicken-Derived Single-Chain Variable Fragments Targeting Monkeypox Virus A29L Protein.

Monkeypox (mpox), a zoonotic disease, has rapidly spread globally, prompting the WHO to declare it a public health emergency. The long incubation period, early symptoms resembling respiratory infections, and diagnostic challenges hinder timely epidemic control and accurate clinical diagnosis. The monkeypox virus (MPXV) encodes the A29L protein, which binds to cellular heparan sulfate to facilitate infection and serves as a target for treatment and diagnostics. Thus, developing effective diagnostic tools and treatments is critically important. In this study, we expressed and purified Escherichi…

Mutational dynamics and phylogenetic insights into monkeypox virus clade I evolution.

The monkeypox virus (MPXV) has evolved from causing sporadic outbreaks to becoming a global public health threat. The re-emergence of clade I in 2023 poses a significant concern. Recently, the first case of the monkeypox clade I variant was reported in India. In this context, the proposed study examines the distinct evolutionary paths of clades I, Ia, and Ib, focusing on their genetic diversity, mutational profiles, and phylogenetic relationships. Clade Ia shows a slightly higher proportion of APOBEC3-type mutations (39.88 %) compared to Clade Ib (39.25 %), which may be influenced by factors s…

Inflammopharmacological perspectives on plant-derived compounds: ınteraction with monkeypox virus receptors.

This study investigates the interaction potential of selected natural compounds derived from medicinal plants with monkeypox virus (MPXV) receptors using molecular docking approaches, with particular emphasis on their possible immunomodulatory and anti-inflammatory relevance. Binding affinities of phytochemicals were compared to the reference compound Cidofovir. Notably, Cucurbitacin I and Cucurbitacin E demonstrated the strongest affinities, surpassing Cidofovir. Among the tested molecules, Cucurbitacin I showed the most favourable interaction profile, followed by Cucurbitacin E, Piperine, Th…

Innate Immune Response to Monkeypox Virus Infection: Mechanisms and Immune Escape.

BACKGROUND: The reemergence of monkeypox virus (Mpox, formerly monkeypox) in 2022 in non-endemic countries has raised significant concerns for global health due to its high transmissibility and mortality rate. A major challenge in combating Mpox is its ability to evade the host's innate immune system, the first line of defense against viral infections. SUMMARY: Mpox encodes various proteins that interfere with key antiviral pathways and mechanisms, such as the nuclear factor kappa B signaling, cytokine production, complement and inflammasome activation, and chemokine binding. These proteins m…

Antivirals for monkeypox virus: Proposing an effective machine/deep learning framework.

Monkeypox (MPXV) is one of the infectious viruses which caused morbidity and mortality problems in these years. Despite its danger to public health, there is no approved drug to stand and handle MPXV. On the other hand, drug repurposing is a promising screening method for the low-cost introduction of approved drugs for emerging diseases and viruses which utilizes computational methods. Therefore, drug repurposing is a promising approach to suggesting approved drugs for the MPXV. This paper proposes a computational framework for MPXV antiviral prediction. To do this, we have generated a new vir

Emerging Monkeypox Virus Sublineage C.1 Causing Community Transmission, Vietnam, 2023.

We studied a community cluster of 25 mpox cases in Vietnam caused by emerging monkeypox virus sublineage C.1 and imported into Vietnam through 2 independent events; 1 major cluster carried a novel APOBEC3-like mutation. Three patients died; all had advanced HIV co-infection. Viral evolution and its potential consequences should be closely monitored.

Phylogeny of Seven Genomes of Monkeypox Virus - Anhui Province, China, 2023.

WHAT IS ALREADY KNOWN ABOUT THIS TOPIC?: Monkeypox, first identified in Africa in 1958, is a zoonotic disease caused by monkeypox virus (MPXV). Since January 2022, a global outbreak of mpox has spread to over 100 non-endemic countries. As of June 30, 2024, there have been 99,176 confirmed cases and 535 probable cases across 116 countries, resulting in 208 deaths. WHAT IS ADDED BY THIS REPORT?: This report presents an analysis of the whole genome sequences of seven MPXVs from Anhui Province. We discovered that, compared to the reference sequence (NC_063383.1), these genomes exhibit 91 nucleoti…

Monkeypox virus-associated meningoencephalitis diagnosed by detection of intrathecal antibody production.

BACKGROUND: In the 2022 mpox-outbreak most patients presented with mild symptoms. Central nervous system (CNS) involvement has previously be … r and confusion. Neuroimaging and CSF analysis indicated encephalitis. The CSF was PCR-negative for monkeypox virus but intrathecal antibody production was detected. He spontaneously improved over a few days course and recovered fully. CONCLUSIONS: This case of mpox-associated encephalitis shows that CNS involvement in mpox infection may have a relatively mild c …

[Protein expression, purification and mouse antiserum preparation of monkeypox virus A23R].

Objective To express the monkeypox virus (MPXV) A23R protein in Escherichia coli and purify by Ni-NTA affinity column, and to prepare mouse antiserum against MPXV A23R. Methods The recombinant plasmid pET-28a-MPXV-A23R was constructed and transformed into Escherichia coli BL21 to induce the expression of A23R protein. After optimizing the conditions of expression, A23R protein was highly expressed. Recombinant A23R protein was purified by Ni-NTA affinity column and identified by Western blot analysis. The purified protein was used to immunize mice for preparing the A23R polyclonal antibody, an…

Ongoing monkeypox virus outbreak, Portugal, 29 April to 23 May 2022.

Up to 27 May 2022, Portugal has detected 96 confirmed cases of monkeypox. We describe 27 confirmed cases (median age: 33 years (range: 22-51); all males), with an earliest symptom onset date of 29 April. Almost all cases (n = 25) live in the Lisbon and Tagus Valley health region. Most cases were neither part of identified transmission chains, nor linked to travel or had contact with symptomatic persons or with animals, suggesting the possible previously undetected spread of monkeypox.

Human-to-Human Transmission of Monkeypox Virus, United Kingdom, October 2018.

In September 2018, monkeypox virus was transmitted from a patient to a healthcare worker in the United Kingdom. Transmission was probably through contact with contaminated bedding. Infection control precautions for contacts (vaccination, daily monitoring, staying home from work) were implemented. Of 134 potential contacts, 4 became ill; all patients survived.

Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques.

To characterize T cell epitopes in monkeypox virus (MPXV) infected rhesus macaques, we utilized IFNγ Elispot assay to screen 400 predicted peptides from 20MPXV proteins. Two peptides from the F8L protein, an analog of E9L protein in vaccinia, were found to elicit CD8+ T cell responses. Prediction and in vitro MHC binding analyses suggest that one is restricted by Mamu-A1(⁎)001 and another by Mamu-A1(⁎)002. The Mamu-A1(⁎)002 epitope is completely identical in all reported sequences for variola, vaccinia, cowpox and MPXV. The Mamu-A1(⁎)001 epitope is conserved in MPXV and vaccinia, and has one r…