Результаты поиска

Variability of the NS(S) protein among Rift Valley fever virus isolates.

Eighteen strains of Rift Valley fever (RVF) virus collected over a period of 38 years and isolated from diverse localities in Africa and from various hosts (human, animal and arthropod) were investigated by RT-PCR followed by sequencing of the NS(S) protein coding region. This region was chosen to analyse variability because, in contrast to the N protein, the NS(S) protein differs in various phleboviruses and there exists an RVF virus (clone 13) in which 70% of the NS(S) ORF is deleted, suggesting that this sequence is under a weak selective pressure. Sequence data indicated that percentage di…

Rift Valley fever: a sero-epidemiological survey among pregnant women in Mozambique.

Rift Valley fever (RVF) causes abortion in sheep and cattle. However, the teratogenic and abortogenic potential of RVF in humans is not known. Sera from a total of 1163 pregnant women in Mozambique were tested for RVF virus antibodies by ELISA and 28 (2%) were found to be positive. Mothers experiencing fetal death or miscarriage (155) had the same RVF virus antibody prevalence as those with normal deliveries. Analysis of maternity histories showed some indication of increased fetal wastage among women positive for RVF virus antibody. The ELISA used in this study was compared with a plaque redu…

Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay.

A double-antibody (sandwich) enzyme-linked immunosorbent assay (ELISA) was adapted to detect Rift Valley fever virus antigen. Antibodies were purified from hyperimmune mouse and rabbit sera by affinity chromatography, using CNBr-activated Sepharose 4B coupled to a beta-propiolactone-inactivated sucrose-acetone-extracted suckling mouse liver antigen. In the assay, antigen was captured by mouse antibody adsorbed to polystyrene plates and then detected by reacting sequentially with rabbit anti-Rift Valley fever virus antibody and swine anti-rabbit immunoglobulin G conjugated to alkaline phosphata…

Marburg, Ebola and Rift Valley Fever virus antibodies in East African primates.

Sera from 464 primates held at four institutes in Kenya were tested by indirect immunofluorescence for the presence of antibodies against Marburg, Ebola, Congo haemorrhagic fever, Rift Valley fever and Lassa viruses. Four of 136 vervet monkeys were positive for Marburg virus antibodies and three of 184 baboons had antibodies against Ebola virus. One baboon was positive for Marburg virus antibodies. Two vervet monkeys, three baboons and one grivet monkey (of 56 tested) had antibodies against Rift Valley fever virus. No Congo or Lassa virus antibodies were detected. A sample of 88 sera of more a…

Rift Valley fever ocular manifestations: observations during the 1977 epidemic in Egypt.

Ocular manifestations resulting from Rift Valley fever (RVF) virus infection were studied during an extensive RVF epidemic in Egypt during 1977. Colour photography and fluorescein angiography of 7 serologically diagnosed patients showed the commonest manifestations to be macular, paramacular, and/or extramacular retinal lesions, often occurring bilaterally. Haemorrhage and oedema were frequently associated with the lesions, and vasculitis, retinitis, and vascular occlusion were also observed. Patients were monitored during a 6-month convalescence, and, though resorption of the lesions occurred…

Integrated surveys of Rift Valley fever, yellow fever and dengue in Lobaye, Central African Republic.

BACKGROUND: Confirmation by plaque reduction neutralization testing (PRNT) of concurrent cases of Rift Valley fever virus (RVFV), yellow fever virus (YFV) and dengue virus (DENV) in Safa village (Lobaye, Central African Republic, CAR) triggered a One Health investigation to assess human, animal, entomological and socio-environmental risk factors for arboviral transmission. METHODS: We conducted integrated epidemiological surveys, blood sampling in humans and domestic animals, larval and adult mosquito collections, and socio-anthropological interviews in Safa and the neighbouring village of Po…

Reversed passive hemagglutination and inhibition with Rift Valley fever and Crimean-Congo hemorrhagic fever viruses.

Reversed passive hemagglutination (RPHA) tests were performed by coating glutaraldehyde-fixed and tannic acid-treated sheep erthrocytes with antibodies to Rift Valley fever (RVF) and Crimean-Congo hemorrhagic fever (CCHF) viruses. Cells coated with crude antibody, in the form of mouse immune ascitic fluid, reacted with high specificity and sensitivity in RPHA tests with live or inactivated virus antigens. In inhibition (RPHI) tests, RVF antibody titers in human and sheep sera were similar to those determined by hemagglutination inhibition test. RVF virus antigen could be detected in viremic sh…

Rift Valley fever virus dynamics in a transhumant cattle system in The Gambia.

Rift Valley fever (RVF) is a zoonotic disease of global concern, driven by environmental conditions, vector activity, and livestock mobility. Although RVF has been reported in The Gambia, its epidemiology remains poorly understood. This study developed a compartmental model to study RVF dynamics in the cattle population of the country. The model incorporated seasonally dynamic transmission parameters reflecting transhumant movement and ecological differences between two distinct ecoclimatic regions: the Sahelian area and the Gambia river. Parameterised using serological data linked to househol…

Rift Valley fever: An emerging zoonotic threat to global health and agriculture.

Rift Valley fever (RVF) is an acute, mosquito-borne zoonotic disease caused by the RVF virus (RVFV), which belongs to the Phlebovirus genus of the Bunyaviridae family. The infection mainly targets domestic ruminants such as sheep, goats, cattle, and camels, with sheep showing the highest vulnerability. The virus is spread predominantly through the bites of Aedes and Culex mosquitoes, although humans may also become infected by direct exposure to blood, organs, or secretions of diseased animals. In livestock, the illness is marked by sudden fever, ocular and nasal discharges, vomiting, and swol…

Rift Valley Fever Virus: An update on current status and future prospects.

Rift Valley fever is a mosquito-borne disease caused by the Rift Valley Fever Virus (RVFV) belonging to the genus Phlebovirus. This virus causes febrile or hemorrhagic illness in humans and ruminants, such as abortion, and death; especially in young sheep, cattle, and goats resulting in devastating epidemics in Africa and the Arabian Peninsula. The WHO has included this virus in Bluepoint's list of eight pathogens. This virus is a crucial health concern in the Kingdom of Saudi Arabia (KSA), as the Kingdom is regularly exposed to this virus from the original source of East African countries. A …

Heat Treatment as a Safe-Handling Procedure for Rift Valley Fever Virus.

Rift Valley Fever virus (RVFV) is a mosquito-borne virus with high pathogenic potential in ruminants and humans. Due to its high potential for spreading, it is considered a priority pathogen, and it is included in the Bluepoint list of the World Health Organization (WHO). Given the high pathogenic potential of the virus, it is crucial to develop a rapid heat-mediated inactivation protocol to create a safer working environment, particularly in medical facilities that lack a biosafety level 3 laboratory required for direct handling of RVFV. Our results reveal the broad tissue tropism of RVFV, sh…

Lrp1 имеет важнейшее значение для смертельного печеночного заболевания мышей в Рифт-Вэлли.

Вирус лихорадки Рифт-Вэлли (RVFV) — формирующийся арбовирус, обнаруженный в Африке. В то время как RVFV носит пантропический характер и заражает многие клетки и ткани, вирусное воспроизведение и некроз внутри печени играют решающую роль в сдерживании серьезных заболеваний. Белок 1 (Lrp1), связанный с рецептором липопротеина с низкой плотностью, является недавно выявленным фактором, определяющим проникновение в клетку и инфекцию с помощью RVFV. Однако биологическая значимость Lrp1, включая ее роль в печени в vivo, еще не определена. Поскольку Lrp1 имеет высокий уровень выражения в гепатоцитах,

Совпадение в аннотации оригинала
Rift Valley fever virus (RVFV) is an emerging arbovirus found in Africa. While RVFV is pantropic and infects many cells and tissues, viral replication and necrosis within the liver play a critical role in mediating severe disease. The low-density lipoprotein receptor-related protein 1 (Lrp1) is a recently identified host factor for cellular entry and infecti…

Исследование иммуногенности и безопасности вакцины против лихорадки Рифт-Вэлли.

Вулканическая лихорадка Рифт-Вэлли (RVF) представляет собой появляющуюся трансграничную, трансмиссивную, зоономическую вирусную болезнь, вызванную одним серотипом вируса, принадлежащего семейству фенуивиридае (ген Флебовирус). Она считается серьезной угрозой как для сельского хозяйства, так и для здоровья населения в эндемических районах, поскольку вирус, передаваемый различными генами комаров, приводит к абортам в уязвимых для здоровья животных местах, особенно в овцах, козах, скоте и буйволах, что приводит к серьезным экономическим потерям. Люди могут также приобрести инфекцию, и основные ис

Совпадение в аннотации оригинала
Rift Valley fever (RVF) is an emerging transboundary, mosquito-borne, zoonotic viral disease caused by a single serotype of a virus belonging to the Phenuiviridae family (genus Phlebovirus). It is considered an important threat to both agriculture and public health in endemic areas, because the virus, transmitted by different mosquito genera, leads to aborti…

No evidence of Rift Valley fever antibodies in veterinarians and sheep in Northern Palestine.

BACKGROUND AND AIM: Rift Valley fever virus (RVFV) is a vector-borne virus that causes RVF in humans and ruminants. The clinical symptoms in humans and animals are non-specific and often misdiagnosed, but abortions in ruminants and high mortality in young animals are characteristic. Since the initial outbreak in the Rift Valley area in Kenya, the disease has spread to most African countries and the Middle East. The presence and epidemiological status of RVFV in humans and animals in Palestine are unknown. This study aimed to investigate the presence and risk factors for RVF seroprevalence in v…

Rift Valley Fever: a Threat to Pregnant Women Hiding in Plain Sight?

The potential for emerging mosquito-borne viruses to cause fetal infection in pregnant women was overlooked until the Zika fever outbreak several years ago. Rift Valley fever virus (RVFV) is an emerging arbovirus with a long history of fetal infection and death in pregnant livestock. The effect of RVFV infection on pregnant women is not well understood. This Gem examines the effects that this important emerging pathogen has during pregnancy, its potential impact on pregnant women, and the current research efforts designed to understand and mitigate adverse effects of RVFV infection during preg…