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Chemometric Methods Applied to Infrared and Raman Spectroscopy for Arboviruses Diagnosis: A Systematic Review With Meta-Analysis.

Arboviruses such as dengue, Zika, chikungunya and yellow fever share similar clinical manifestations, making differential diagnosis challenging, particularly in endemic regions with viral co-circulation. Conventional laboratory methods present important limitations, including cross-reactivity and reliance on specialized infrastructure. In this scenario, spectroscopic techniques such as Fourier-transform attenuated total reflectance infrared spectroscopy (ATR-FTIR) and Raman, when combined with artificial intelligence (AI), have shown promise by enabling rapid, low-cost analyses. This systemati…

Development of nucleic acid-based vaccines against dengue and other mosquito-borne flaviviruses: the past, present, and future.

Due to their widespread geographic distribution and frequent outbreaks, mosquito-borne flaviviruses, such as DENV (DENV), Zika virus (ZIKV), Japanese encephalitis virus (JEV), yellow fever virus (YFV), and West Nile virus (WNV), are considered significant global public health threats and contribute to dramatic socioeconomic imbalances worldwide. The global prevalence of these viruses is largely driven by extensive international travels and ecological disruptions that create favorable conditions for the breeding of Aedes and Culex species, the mosquito vectors responsible for the spread of thes…

Anti-NMDA receptor encephalitis following Murray Valley encephalitis: a case report.

BACKGROUND: Anti-N-methyl-D-aspartate (NMDA) receptor encephalitis is an autoimmune condition that is increasingly being recognized as a complication of central nervous system infections. While association with herpes simplex virus encephalitis is well established, the condition has also been reported to occur following Japanese encephalitis virus infection and yellow fever vaccination. CASE SUMMARY: Here, we report a case of anti-NMDA receptor encephalitis following infection with Murray Valley encephalitis virus (MVEV), another virus of the family Flaviviridae. Following initial recovery, a…

Spatial-temporal analysis of natural hazards and disasters in the Greater Horn of Africa between 2010 and 2024 to inform disaster risk reduction, and surveillance and control strategies for climate and environmentally sensitive diseases.

OBJECTIVE: To determine the spatial-temporal patterns of natural hazards and disasters in the Greater Horn of Africa, including climate and … 11 storms and 17 landslides; 46 epidemics reported, primarily bacterial (eg, cholera) or viral (eg, yellow fever, measles) diseases, with 20% preceded by a flood, drought or landslide within the previous 3 months. Reporting consistency and content varied considerably across the five databases. CONCLUSION: Natural hazards and disasters affect millions of peopl …

Global, asynchronous partial sweeps at multiple insecticide resistance genes in Aedes mosquitoes.

Aedes aegypti (yellow fever mosquito) and Ae. albopictus (Asian tiger mosquito) are globally invasive pests that confer the world's dengue burden. Insecticide-based management has led to the evolution of insecticide resistance in both species, though the genetic architecture and geographical spread of resistance remains incompletely understood. This study investigates partial selective sweeps at resistance genes on two chromosomes and characterises their spread across populations. Sweeps at the voltage-sensitive sodium channel (VSSC) gene on chromosome 3 correspond to one resistance-associated…

Сравнительная оценка выборочных серологических анализов вируса Зика с использованием слепых справочных групп.

В ответ на эпидемию вируса Zika (ZIKV) 2015 года, имевшую место в Бразилии, были подготовлены многочисленные коммерческие серологические анализы для применения в клинических и исследовательских целях. Диагноз недавней инфекции у беременных женщин по-прежнему остается непростым. Благодаря стандартизации сравнительные исследования тестов ZIKV имеют важное значение для проведения оптимальных диагностических тестов и наблюдения за заболеваниями. Это особенно важно для серологических тестов, используемых для обнаружения инфекции ZIKV, с учетом того, что антитела против ZIKV могут перекликаться с др

Совпадение в аннотации оригинала
In response to the 2015 Zika virus (ZIKV) epidemic that occurred in Brazil, numerous commercial serological assays have been developed for c … ZIKV can cross-react with other arboviruses in the same virus family, such as dengue virus (DENV), yellow fever virus (YFV) and West Nile virus (WNV). We looked at the sensitivity and specificity of tests detecting ZIKV antibodies (IgM, IgG) from multiple manufacturers using panels of samples previously collected with known exposure to ZIKV and other arboviru …

Minimizing Vaccine Wastage in Nigeria: A National Assessment of Vaccine Wastage Rates and Potential Determinants.

High vaccine wastage can serve as a critical barrier to achieving the gains of vaccination, especially in a country like Nigeria, where data … lent vaccine to 72.6% (95% CI: 71.5%, 73.6%) for BCG. Open-vial wastage rates for BCG, measles, and yellow fever vaccines were higher during outreach sessions, with rates of 76.2%, 64.3%, and 65.2%, respectively. For the outreach and fixed sessions, PCV and Penta had the least wastages of 22.0% for fixed sessions and 20.4% for outreach sessions. This study ide …

Естественный отбор формирует использование кодона и адаптирует NS1 в патогенных флавиирусах, переносимых комарами.

Протеин NS1 из девяти флавивирусов, передающихся через комары, включая вирус Денге 1-4, японский энцефалит, вирус Западного Нила, вирус Желтой лихорадки, вирус Тембусу и вирус Зика, демонстрирует явное использование кодона и эволюционные черты. Анализ использования кодона показывает заметные отклонения в составе и отсутствие консерватизма в NS1 с отличительными эволюционными чертами от его ОРФ. Анализ относительно синонимов использования кодона (RSCU) показывает, что гены NS1 имеют неконсервативные модели RSCU в различных патогенных флавивирусах, переносимых комарами. Анализ основных компонент

Совпадение в аннотации оригинала
The NS1 protein of nine mosquito-borne flaviviruses, including Dengue virus 1-4, Japanese encephalitis virus, West Nile virus, Yellow fever virus, Tembusu virus, and Zika virus, shows distinct codon usage and evolutionary traits. Codon usage analysis shows notable base composition bias and non-conservatism in NS1, with distinct evolutionary traits from its O…

Strong Positive Selection in Aedes aegypti and the Rapid Evolution of Insecticide Resistance.

Aedes aegypti vectors the pathogens that cause dengue, yellow fever, Zika virus, and chikungunya and is a serious threat to public health in tropical regions. Decades of work has illuminated many aspects of Ae. aegypti's biology and global population structure and has identified insecticide resistance genes; however, the size and repetitive nature of the Ae. aegypti genome have limited our ability to detect positive selection in this mosquito. Combining new whole genome sequences from Colombia with publicly available data from Africa and the Americas, we identify multiple strong candidate sele…

Evaluation of Brown and red seaweeds-extracts as a novel larvicidal agent against the deadly human diseases-vectors, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus.

Infectious diseases such as malaria, dengue, and yellow fever are predominantly transmitted by insect vectors like Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus in tropical regions like India and Africa. In this study, we assessed the larvicidal activity of commonly found seaweeds, including Padina gymnospora, P. pavonica, Gracilaria crassa, Amphiroa fragilissima, and Spatoglossum marginatum, against these mosquito vectors. Our findings indicate that extracts from P. gymnospora Ethyl Acetate (PgEA), P. pavonica Hexane (PpH), and A. fragilissima Ethyl Acetate (AfEA) displayed t…

Novel molecular approaches to combat vectors and vector-borne viruses: Special focus on RNA interference (RNAi) mechanisms.

Vector-borne diseases, such as dengue, chikungunya, zika, yellow fever etc pose significant burden among the infectious diseases globally, especially in tropical and sub-tropical regions. Globalization, deforestation, urbanization, climate change, uncontrolled population growth, inadequate waste management and poor vector-management infrastructure have all contributed to the expansion of vector habitats and subsequent increase in vector-borne diseases throughout the world. Conventional vector control methods, such as use of insecticides, have significant negative environmental repercussions in…

Analysis of Aedes aegypti microRNAs in response to Wolbachia wAlbB infection and their potential role in mosquito longevity.

The mosquito Aedes aegypti is the primary vector of a range of medically important viruses including dengue, Zika, West Nile, yellow fever, and chikungunya viruses. The endosymbiotic bacterium Wolbachia pipientis wAlbB strain is a promising biocontrol agent for blocking viral transmission by Ae. aegypti. To predict the long-term efficacy of field applications, a thorough understanding of the interactions between symbiont, host, and pathogen is required. Wolbachia influences host physiology in a variety of ways including reproduction, immunity, metabolism, and longevity. MicroRNAs (miRNAs) are …

Computational Approaches in the Discovery and Development of Therapeutic and Prophylactic Agents for Viral Diseases.

Over the last two decades, computational technologies have played a crucial role in antiviral drug development. Whenever a virus spreads and … putational methods in developing small molecules and vaccines against human immunodeficiency virus, yellow fever, human papilloma virus, SARS-CoV-2, and other viruses. Various computational tools available for the abovementioned purposes have been listed and described. A discussion on applying artificial intelligence-based methods for antiviral drug discovery …

Facile synthesis, antimicrobial and antiviral evaluation of novel substituted phenyl 1,3-thiazolidin-4-one sulfonyl derivatives.

A series of novel substituted phenyl 1, 3-thiazolidin-4-one sulfonyl derivatives 5 (a-t) were synthesized and screened for their in-vitro an … KOS ACVr), Vaccinia virus, Human Coronavirus (229E), Reovirus-1, Sindbis virus, Coxsackie virus B4, Yellow Fever virus and Influenza A, B virus. Compounds 5h exhibited low anti-viral activity against HIV-1(strain IIIB) and HIV-2 (strain ROD). The study clearly outlined that synthesized compounds endowed with good antimicrobial property together with considerab …

Zika virus subversion of chaperone GRP78/BiP expression in A549 cells during UPR activation.

Flaviviruses replicate in membranous factories associated with the endoplasmic reticulum (ER). Significant levels of flavivirus polyprotein … responses, all three pathways leading to UPR are activated during infection by Dengue virus (DENV), Yellow Fever virus (YFV) or West Nile virus (WNV). In the present study, we investigated the capacity of ZIKA virus (ZIKV) to induce ER stress in A549 cells. We observed that the cells respond to ZIKV infection by implementing an UPR through activation of the IR …

Ross River Virus Provokes Differentially Expressed MicroRNA and RNA Interference Responses in Aedes aegypti Mosquitoes.

Alphaviruses are globally distributed and predominately transmitted by mosquitoes. Aedes species are common vectors for the clinically important alphaviruses-Chikungunya, Sindbis, and Ross River (RRV) viruses-with Aedes aegypti also being a vector for the flaviviruses dengue, Yellow Fever, and Zika viruses. Ae. aegypti was putatively implicated in the large 1979-1980 South Pacific Islands outbreak of RRV-the leading cause of arboviral disease in Australia today. The RNA interference (RNAi) defense response in mosquitoes involves a number of small RNAs, with their kinetics induced by alphavirus…

Downregulation of IGF2 expression in third trimester placental tissues from Zika virus infected women in Brazil.

OBJECTIVES: Screening for genes differentially expressed in placental tissues, aiming to identify transcriptional signatures that may be inv … ion in Caco-2 cells, whereas other flaviviruses, such as dengue serotype 1, West Nile and wild-type yellow fever viruses, had no effect on this gene expression. Immunohistochemical assays on decidual tissues corroborated our transcriptome analysis, showing that IGF2 is reduced in the decidua of Zika virus-infected women. CONCLUSIONS: Our results draw attentio …

Zika Virus-Like Particle (VLP) vaccine displaying Envelope (E) protein CD loop antigen elicits protective and specific immune response in a murine model.

Zika virus (ZIKV) is a mosquito-borne flavivirus associated with Congenital Zika Syndrome (CZS), reflecting a wide range of congenital abnor … Th2- mediated immune response. No cross-reacting antibodies were detected between Zika, dengue and yellow fever virus, demonstrating a high level of specificity for the ZIKV CD Loop antigen. Immunization with the WHcAg CD Loop vaccine candidate demonstrated immunoprotection in a murine model of ZIKV infection, stimulating protective antibodies associated with …

Выяснить оптимальные сроки дублирования для стабильности репортеров.

Флавивирусы, передающиеся с помощью москито, вызывают широко распространенные болезни во всем мире. В целях обеспечения более совершенных молекулярных инструментов для изучения лекарственных экранов и патогенеза мы сообщаем о новом подходе к производству стабильных флавивирусов, пропитанных нанолуком, включая серотипы денге 1-4, японский энцефалит, вирус желтой лихорадки, вирус Западного Нила и вирус Зика. Поскольку ген репортера часто проектируется в генной области Капсида, последовательность капсида должна быть дублирована для фланга гена репортера; такое копирование капсида имеет важное зна

Совпадение в аннотации оригинала
ABSTRACT Mosquito-transmitted flaviviruses cause widespread disease across the world. To provide better molecular tools for drug screens and pathogenesis studies, we report a new approach to produce stable NanoLuc-tagged flaviviruses, including dengue virus serotypes 1-4, Japanese encephalitis virus, yellow fever virus, West Nile virus, and Zika virus. Since…

Climate change may enable Aedes aegypti infestation in major European cities by 2100.

BACKGROUND: Climate change allows Aedes aegypti to infest new areas. Consequently, it enables the arboviruses the mosquito transmits -- e.g., dengue, chikungunya, Zika and yellow fever - to emerge in previously uninfected areas. An example is the Portuguese island of Madeira during 2012-13. OBJECTIVE: We aim to understand how climate change will affect the future spread of this potent vector, as an aid in assessing the risk of disease outbreaks and effectively allocating resources for vector control. METHODS: We used an empirically-informed, process-based mathematical model to study the feas…