Результаты поиска

Fatal Canine Distemper Virus Infections in Free-Ranging Black-Tufted Marmosets (Callithrix penicillata) in Central Brazil.

Canine distemper virus (CDV), a morbillivirus with a broad host range, has increasingly been reported in wildlife, including nonhuman primat … demonstrated multifocal CDV antigen labeling in pulmonary and esophageal tissues, whereas tests for yellow fever virus, human alphaherpesvirus, and Toxoplasma gondii were negative. Molecular analysis confirmed CDV infection by amplification of short fragments of the conserved L gene, which showed ≥98% nucleotide identity with Brazilian strains. However, furthe …

A Review of Arboviral-Induced Liver Damage: Acute Manifestations and Speculative Links to Chronic Inflammation.

Arboviruses are a heterogeneous group of vector-borne viruses from various viral families. The clinical manifestations associated with these … liver involvement during acute arboviral infections, including those caused by Dengue Virus (DENV), Yellow Fever Virus (YFV), Zika Virus (ZIKV), and Chikungunya Virus (CHIKV). While much of the available data pertains to acute liver damage, such as H, hepatomegaly, or fulminant hepatitis, it also discusses the possibility that such acute injuries may, in certa …

A Rapid On-Site Detection Method for Plasmodium falciparum Carried by Mosquitoes Using Disc Microfluidic Isothermal Amplification.

Rapidly identifying Anopheles-carrying malaria parasites is crucial for imported malaria prevention. However, suitable methods still lack qu … of Anopheles mosquitoes and morphologically similar arthropods or with the copy DNA of Zika virus, yellow fever virus, or dengue virus 1. The detection method was evaluated in a simulated scenario and demonstrated a robust capacity for rapid on-site detection. Additionally, polymerase chain reaction (PCR) and quantitative real-time PCR methods were compared u …

Репортер по серонейтрализации частиц вируса тикает энцефалит, преодолевая ограничения ELISA.

Вирус энцефалита (TBE) — самый распространенный в Европе ортофлавирус, передаваемый клещами. В силу неконкретного характера симптомов ТБЭ диагностируется главным образом по обнаружению конкретных антител в сыворотке пациента на основе ЭЛИСА. Однако перекрестная реактивность между ортофлавиирусами затрудняет диагностику. Вопросы специфичности могут быть смягчены с помощью нейтрализации сыворотки (SNT), хотя для обработки клинически значимых орфолавиирузов требуются условия уровня 3 по биобезопасности (BSL) и они характеризуются весьма различными вирусными кинетиками и трпизами клеток. В настоящ

Совпадение в аннотации оригинала
Tick-borne encephalitis (TBE) virus is the most prevalent tick-transmitted orthoflavivirus in Europe. Due to the nonspecific nature of its s … o tested the cross-reactivity of serum samples in RVP-based assays against other orthoflaviviruses (yellow fever virus, dengue virus type 2, Zika virus, West Nile virus and Japanese encephalitis virus). Interestingly, all serum samples which had tested TBEV-positive by ELISA but negative by RVP-based SNT were reactive for antibodies against other orthoflavivir …

Niche and Range Shifts of Aedes aegypti and Ae. albopictus Suggest That the Latecomer Shows a Greater Invasiveness.

The yellow fever (Aedes aegypti) and Asian tiger (Ae. albopictus) mosquitos are major vectors of global mosquito-borne pathogens. However, their niche and range shifts, the underlying mechanisms, and related relative invasion rates remain scarcely known. We examined the niche and range shifts between the native and invasive Ae. aegypti and Ae. albopictus populations through dynamic niche and range models and the largest occurrence record datasets to date. We detected substantial niche and range expansions in both species, probably because the introduced populations have more opportunities to a…

Rapid Generation of Recombinant Flaviviruses Using Circular Polymerase Extension Reaction.

The genus Flavivirus is a group of arthropod-borne single-stranded RNA viruses, which includes important human and animal pathogens such as Japanese encephalitis virus (JEV), Zika virus (ZIKV), Dengue virus (DENV), yellow fever virus (YFV), West Nile virus (WNV), and Tick-borne encephalitis virus (TBEV). Reverse genetics has been a useful tool for understanding biological properties and the pathogenesis of flaviviruses. However, the conventional construction of full-length infectious clones for flavivirus is time-consuming and difficult due to the toxicity of the flavivirus genome to E. coli. …

Факторы риска, связанные с непрививкой детей Гамбии: исследование групп населения.

СПРАВОЧНАЯ ИНФОРМАЦИЯ: Мы определили факторы риска, связанные с непрививаемыми детьми в сельских районах Гамбии. МЕТОДЫ: Мы провели перспективное демографическое наблюдение и зарегистрировали вакцинацию в режиме реального времени в районе Верхней реки, Гамбия. Анализ охватывал жителей, родившихся с 1 января 2012 года по 31 декабря 2016 года. Данные включают данные о возрасте, поле, членах домашнего хозяйства и взаимоотношениях, миграции, рождении, смерти, этнической принадлежности, месте проживания и типе рождения. Дети были определены как не вакцинированные в возрасте 10, 15 и 24 месяцев, есл

Совпадение в аннотации оригинала
BACKGROUND: We determined the risk factors associated with unvaccinated children in rural Gambia. METHODS: We conducted prospective demogra … tavalent, oral polio and pneumococcal conjugate vaccines), secondary series (first dose measles and yellow fever vaccines) or both vaccination series, respectively. Logistic regressions measured the association between risk factors and being unvaccinated. RESULTS: In total, 5% (1567/30 832) of infants born during the study period and who were residents at the …

Olfaction-Related Gene Expression in the Antennae of Female Mosquitoes From Common Aedes aegypti Laboratory Strains.

Adult female mosquitoes rely on olfactory cues like carbon dioxide and other small molecules to find vertebrate hosts to acquire blood. The molecular physiology of the mosquito olfactory system is critical for their host preferences. Many laboratory strains of the yellow fever mosquito Aedes aegypti have been established since the late 19th century. These strains have been used for most molecular studies in this species. Some earlier comparative studies have identified significant physiological differences between different laboratory strains. In this study, we used a Y-tube olfactometer to de…

Transcriptional profiling and physiological roles of Aedes aegypti spermathecal-related genes.

BACKGROUND: Successful mating of female mosquitoes typically occurs once, with the male sperm being stored in the female spermatheca for every subsequent oviposition event. The female spermatheca is responsible for the maintenance, nourishment, and protection of the male sperm against damage during storage. Aedes aegypti is a major vector of arboviruses, including Yellow Fever, Dengue, Chikungunya, and Zika. Vector control is difficult due to this mosquito high reproductive capacity. RESULTS: Following comparative RNA-seq analyses of spermathecae obtained from virgin and inseminated females, …

Insecticidal potential of Brevibacillus laterosporus against dipteran pest species in a wide ecological range.

In order to increase our understanding of the insecticidal potential of the entomopathogenic bacterium Brevibacillus laterosporus strain UNISS 18 against insect pests, investigations were conducted on a selection of dipteran species including fruit flies, house flies, blow flies, and mosquitoes, characterized by adaptations to very diverse habitats. According to lethal concentration (LC50) values, the common house mosquito Culex pipiens (LC50 = 0.10 × 106 spores/mL) and the yellow fever mosquito Aedes aegypti (LC50 = 0.18 × 106 spores/mL) were significantly more susceptible than the flies. The…

Insect-specific viruses and arboviruses in adult male culicids from Midwestern Brazil.

Viruses were identified from male anthropophilic mosquitoes from Mato Grosso (MT) State, Midwest Brazil from February 2017 to January 2018. … ols positive for Ilhéus (1 pool), dengue serotype 4 (1), Mayaro (12), equine encephalitis virus (1) yellow fever (1), Oropouche (2), Zika (4) and chikungunya (12) viruses. High throughput sequencing of arbovirus positive pools identified 35 insect-specific viruses (ISVs) from the families Circoviridae (2), Parvoviridae (2), Totiviridae (1), Flaviviridae (1), I …

A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins.

Genus Flavivirus, which includes 53 virus species, is the leading cause of arthropod-borne diseases in humans. Diagnosis of these viral dise … lope (E) and non-structural 1 (NS1) proteins of different flaviviruses (Zika (ZIKV), Dengue (DENV), Yellow Fever (YFV), West Nile (WNV), Japanese Encephalitis (JEV) and Tick-Borne Encephalitis (TBEV)) and the envelope protein of Chikungunya virus (CHIKV). Using 54 samples from 40 individuals with ZIKV infection that had been pre-characterized, we identified 1) …

Electrophysiological Responses of Eighteen Species of Insects to Fire Ant Alarm Pheromone.

Olfaction plays a dominant role in insect communication. Alarm pheromones, which alert other insects of the same species of impending danger … ix ant species and one hybrid ant, honeybee, bagrada bug, lady beetle, housefly, small hive beetle, yellow fever mosquito, termite, bedbug, water hyacinth weevil, southern green stink bug and two aphid species. In contrast, IPA elicited significant EAG response only in the honeybee, red imported fire ant, an Aphaenogaster ant, and the water hyacinth weevil. Th …

Anti-inflammatory Compound Shows Therapeutic Safety and Efficacy against Flavivirus Infection.

Flaviviruses comprise several medically important viruses, including Japanese encephalitis virus, West Nile virus, dengue virus (DENV), yellow fever virus, and Zika virus (ZIKV). A large outbreak of DENV and ZIKV occurred recently, leading to many cases of illness and death. However, despite decades of effort, we have no clinically specific therapeutic drugs against DENV and ZIKV. Previous studies showed that inflammatory responses play a critical role in dengue and Zika virus pathogenesis. Thus, in this study, we examined a series of novel anti-inflammatory compounds and found that treatment …

Parental and offspring larval diets interact to influence life-history traits and infection with dengue virus in Aedes aegypti.

The environmental conditions experienced by parents can influence offspring phenotype along with the conditions experienced by offspring. These parental effects are clear in organisms that display parental care and are less clear in other organisms. Here, we consider effects of parental and offspring larval nutrition on offspring development time, survivorship and infection with dengue virus in Aedes aegypti, the mosquito vector of dengue, chikungunya, yellow fever and Zika. Parents were raised on either high or low larval detritus inputs with subsequent offspring being divided into two groups…

Tree hole mosquito species composition and relative abundances differ between urban and adjacent forest habitats in northwestern Argentina.

Water-holding tree holes are main larval habitats for many pathogen vectors, especially mosquitoes (Diptera: Culicidae). Along 3 years, the … ned presence of Ae. aegypti, Haemagogus and Sabethes in the area also highlight a potential risk of yellow fever epidemics. Overall results show an impoverished tree hole mosquito fauna in urban environments, reflecting negative effects of urbanization on mosquito diversity.

A first case report of rare synchronous double cancers: malignant cutaneous melanoma and gastrointestinal stromal tumor.

Serological diagnosis of Zika virus (ZIKV) infections is challenging due to high cross-reactivity between flaviviruses. We evaluated the dia … litis virus (n = 25), chikungunya virus (n = 19) or Plasmodium spp. (n = 69) infections and from 12 yellow fever-vaccinated individuals were also examined. In confirmed ZIKV specimens collected ≥ 6 days after symptom onset, ELISA sensitivity was 58.8% (95% confidence interval (CI): 36.0-78.4) for IgM, 88.2% (95% CI: 64.4-98.0) for IgG, and 100% (95% CI: 78.4-1 …

Dengue-associated maculopathy.

OBJECTIVE: To describe the clinical spectrum of fundus manifestations and angiographic and optical coherence tomographic features of dengue-associated maculopathy in a large series. METHODS: We reviewed clinical records of patients diagnosed as having dengue maculopathy at the Singapore National Eye Centre between January 1, 2002, and December 31, 2005. RESULTS: We identified 41 patients with serological evidence of dengue fever who had ocular signs and symptoms not attributable to other diseases within 1 month after onset of symptoms of dengue. Seventy-one eyes had maculopathy. Mean best-co

Antibodies against West Nile Virus nonstructural protein NS1 prevent lethal infection through Fc gamma receptor-dependent and -independent mechanisms.

The flavivirus nonstructural protein NS1 is a highly conserved secreted glycoprotein that does not package with the virion. Immunization with NS1 elicits a protective immune response against yellow fever, dengue, and tick-borne encephalitis flaviviruses through poorly defined mechanisms. In this study, we purified a recombinant, secreted form of West Nile virus (WNV) NS1 glycoprotein from baculovirus-infected insect cells and generated 22 new NS1-specific monoclonal antibodies (MAbs). By performing competitive binding assays and expressing truncated NS1 proteins on the surface of yeast (Saccha…

[Study on the application of geographic information system in spatial distribution of hemorrhage fever with renal syndrome in China].

OBJECTIVE: To analyze the spatial distribution of hemorrhagic fever with renal syndrome (HFRS) in China by geographic information system, and to draw up a map on HRFS risk areas. METHODS: A set of database was set up using the information collected and linked to electronic maps of China in a software ArcGIS 8.01 from 41 HFRS surveillance sites during 1995 - 1998. A HFRS spatial distribution model was developed using inverse distance weighted interpolation of ArcGIS's spatial analysis method. The normalized difference vegetation index (NDVI) in each HFRS surveillance site was extracted from SP