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Research trends in Rift Valley fever virus: a bibliometric analysis from 1936 to 2024.

BACKGROUND: Rift Valley fever (RVF), first identified in Kenya in 1930, is a viral zoonosis transmitted by arthropods and poses significant risks to both public and animal health. Despite the wealth of published studies on the disease, the number of bibliometric analysis of Rift Valley fever virus (RVFV) is limited. This study utilized bibliometric analysis to identify research hotspots and emerging trends in RVFV studies, aiming to offer new insights and strategic references for future research directions and prevention strategies. METHODS: The study utilized Scopus to collect global publica…

Identification and Characterization of a Metal Binding-Independent Rift Valley Fever Virus Endonuclease Inhibitor.

The viral cap-snatching mechanism, facilitated by the endonuclease (EndoN) domain of viral polymerases, is critical for viral gene transcription and translation and is therefore an attractive target for antiviral development. The successful development of EndoN inhibitor XOFLUZA (Baloxavir marboxil) has opened the possibility that the EndoN domain of negative-sense RNA viruses (NSVs) can be targeted in a similar fashion. Rift Valley Fever Virus (RVFV) belongs to the Bunyaviricetes class, which includes other pathogens with pandemic potential, such as severe fever with thrombocytopenia syndrome…

Baculovirus-mediated production and purification of ferritin nanoparticles for rift valley fever vaccine development.

BACKGROUND: Rift Valley fever (RVF) is a WHO-prioritized zoonotic, vector-borne disease with no licensed prophylaxis available for humans, highlighting the need for effective vaccine strategies. Nanoparticle-based platforms for antigen presentation offer a promising approach for vaccine development. RESULTS: In this work, we engineered ferritin (Ft) nanoparticles to display the immunogenic Gn domain of RVF virus (GnFt) and systematically assessed the production, purification, and physico-chemical properties of the purified nanoparticles. Baculovirus-based expression systems were evaluated in …

An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines.

INTRODUCTION: This immunoinformatic study identified potential epitopes from the envelopment polyprotein (Gn/Gc) of Rift Valley fever virus (RVFV), a pathogenic virus causing severe fever in humans and livestock. Effective vaccination is crucial for controlling RVFV outbreaks. The identification of suitable epitopes is crucial for the development of safe and effective vaccines. METHODOLOGY: Protein sequences were obtained from the UniProt database, and evaluated through VaxiJen v2.0 to predict the B and T-cell epitopes within the RVFV glycoprotein. Gn/Gc protein sequences were analyzed with b…

Моделирование эффективности адресной вакцинации от вируса лихорадки Рифт-Вэлли с использованием несовершенной сетевой информации.

Движение скота способствует распространению ряда инфекционных заболеваний. Таким образом, данные о перемещениях скота могут использоваться в качестве руководства для разработки политики в отношении адресных мер по борьбе с инфекционными заболеваниями скота, включая лихорадку Рифт-Вэлли (RVF) - предотвратимую с помощью вакцин арбовирусную лихорадку. Как известно, подробные данные о перемещениях скота являются полезными для адресных усилий по контролю, включая вакцинацию. Эти данные имеются во многих странах, однако такие данные, как правило, отсутствуют в других странах, в том числе во многих с

Совпадение в аннотации оригинала
Livestock movements contribute to the spread of several infectious diseases. Data on livestock movements can therefore be harnessed to guide policy on targeted interventions for controlling infectious livestock diseases, including Rift Valley fever (RVF)-a vaccine-preventable arboviral fever. Detailed livestock movement data are known to be useful for target…

Seroprevalence of Rift Valley Fever virus in one-humped camels (Camelus dromedaries) in Egypt.

Rift Valley fever (RVF) is a mosquito-borne viral disease that affects a variety of domestic animals, including cattle, sheep, goats, and camels, and has zoonotic potential. Although the rift valley fever virus (RVFV) is usually asymptomatic in camels, it can induce abortion in some pregnant animals. In the current study, a serosurvey was carried out to investigate the prevalence of RVFV antibodies and related risk factors in camels from four Egyptian governorates. A total of 400 serum samples were examined for anti-RVFV antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA…

First detection of Rift Valley fever virus antibodies in non-human primates in Cameroon.

We tested for Rift Valley fever virus (RVFV) from at least 15 species of non-human primates. RVFV IgG/IgM antibodies were detected in 3.7% (2 out of 53) of chimpanzees (Pan troglodytes) and in 1.4% (1 out of 72) of unidentified non-human primate species. This study was the first investigation of RVFV in monkeys in Cameroon.

An outbreak of Rift Valley fever among peri-urban dairy cattle in northern Tanzania.

BACKGROUND: Human and animal cases of Rift Valley fever (RVF) are typically only reported during large outbreaks. The occurrence of RVF cases that go undetected by national surveillance systems in the period between these outbreaks is considered likely. The last reported cases of RVF in Tanzania occurred during a large outbreak in 2007-2008. METHODS: Samples collected between 2017 and 2019 from livestock suffering abortion across northern Tanzania were retrospectively tested for evidence of RVF virus infection using serology and reverse transcription quantitative polymerase chain reaction (RT…

Rift Valley fever MP-12 vaccine elicits an early protective immune response in mice.

Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen that causes outbreaks of severe disease in people and livestock throughout Africa and the Arabian Peninsula. The development of an effective veterinary and human vaccine to protect against Rift Valley fever (RVF) disease remains a high priority. The live attenuated RVFV MP-12 is a promising vaccine candidate for the prevention of RVF in both human and domestic ruminants. The aim of this study was to determine the onset of protective immunity elicted in mice by a single dose of this vaccine. Groups of CD-1 mice were vaccinat…

Potent neutralization of Rift Valley fever virus by human monoclonal antibodies through fusion inhibition.

Rift Valley fever virus (RVFV), an emerging arboviral and zoonotic bunyavirus, causes severe disease in livestock and humans. Here, we report the isolation of a panel of monoclonal antibodies (mAbs) from the B cells of immune individuals following natural infection in Kenya or immunization with MP-12 vaccine. The B cell responses of individuals who were vaccinated or naturally infected recognized similar epitopes on both Gc and Gn proteins. The Gn-specific mAbs and two mAbs that do not recognize either monomeric Gc or Gn alone but recognized the hetero-oligomer glycoprotein complex (Gc+Gn) whe…

Производство и характеристика моноклональных антител против N Protein из Вируса лихорадки Рифт-Вэлли.

Осколки ДНК, кодирующие основной антигенный район, аа 14-245 на N-белке вируса Рифт-Вэлли (RVFV), были клонированы в вектора PET-28a (+) и p3xFLAG-CMV-10. Рекомбинантный белок PET-28a-N1 был выращен в Escherichia coli BL21 (DE3) с 1 мм изоппил-б-тио-галактопиранасид при температуре 37°C в течение 5 часов и очищен белковым очищателем. Три моноклональных антитела (mAbs) под названием 3A5, 3A6 и 3A7 против N-белка были получены с помощью взрывателя клеточной линии мыши миэломы SP2/0 с селезенками лимфоцитов из мышей PET-28a-N1 с белковым иммунизированными мышами. И наконец, MAb были отмечены имму

Rift Valley fever virus targets the maternal-foetal interface in ovine and human placentas.

BACKGROUND: Rift Valley fever virus (RVFV) is an arbovirus of the order Bunyavirales that causes severe disease in ruminants and humans. Outbreaks in sheep herds are characterised by newborn fatalities and abortion storms. The association of RVFV infections with abortions of ovines and other ruminants is well recognized, whereas the pathology resulting in abortion has remained undescribed. Accumulating evidence suggests that RVFV is abortogenic in humans as well, warranting more research on the interaction of RVFV with the ruminant and human placenta. METHODOLOGY/PRINCIPAL FINDINGS: Pregnant …

Immunogenicity Assessment of Rift Valley Fever Virus Virus-Like Particles in BALB/c Mice.

Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV) and is spread by arthropod vectors. Virus-like particle (VLP) vaccines, which have the advantages of strong immunogenicity and safety, play an important role in the prevention of this disease. VLPs for RVFV were successfully prepared by our research group using a baculovirus-insect cell expression system. To study the immunogenicity of these RVFV VLPs, a correct 3rd or 4th generation recombinant baculovirus, rBac-N-G, was identified and used to infect Sf9 cells, which were cultured in suspensio…